Clinical relevance of Mycobacterium tuberculosis plcD gene mutations

Zhenhua Yang, Dong Yang, Ying Kong, Lixin Zhang, Carl F. Marrs, Betsy Foxman, Joseph H. Bates, Frank Wilson, M. Donald Cave

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To identify Mycobacterium tuberculosis virulence factors, we integrated comparative genomics and epidemiologic data analysis to investigate the relationship between certain genomic insertions and deletions in the phospholipase-C gene D (plcD) with the clinical presentation of tuberculosis (TB). Four hundred ninety-six well-characterized M. tuberculosis clinical isolates were studied. Approximately 30% (147) of the isolates had an interruption of the plcD gene. Patients infected with the plcD mutant were twice as likely to have extrathoracic disease as those infected by a strain without an interruption (adjusted odds ratio, 2.19; 95% confidence interval, 1.27, 3.76). When we limited the analysis to the 275 isolates with distinct DNA fingerprint patterns, we observed the same association (adjusted odds ratio, 2.74; 95% confidence interval, 1.35, 5.56). Furthermore, the magnitude of the association appeared to differ with the type of extrathoracic TB. Our findings suggest that the plcD gene of M. tuberculosis is potentially involved in the pathogenesis of TB, and the clinical presentation of the disease may be influenced by the genetic variability of the plcD region.

Original languageEnglish (US)
Pages (from-to)1436-1442
Number of pages7
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume171
Issue number12
DOIs
StatePublished - Jun 15 2005

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Type C Phospholipases
Mycobacterium tuberculosis
Mutation
Genes
Tuberculosis
Odds Ratio
Confidence Intervals
DNA Fingerprinting
Virulence Factors
Genomics

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Clinical relevance of Mycobacterium tuberculosis plcD gene mutations. / Yang, Zhenhua; Yang, Dong; Kong, Ying; Zhang, Lixin; Marrs, Carl F.; Foxman, Betsy; Bates, Joseph H.; Wilson, Frank; Cave, M. Donald.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 171, No. 12, 15.06.2005, p. 1436-1442.

Research output: Contribution to journalArticle

Yang, Z, Yang, D, Kong, Y, Zhang, L, Marrs, CF, Foxman, B, Bates, JH, Wilson, F & Cave, MD 2005, 'Clinical relevance of Mycobacterium tuberculosis plcD gene mutations', American Journal of Respiratory and Critical Care Medicine, vol. 171, no. 12, pp. 1436-1442. https://doi.org/10.1164/rccm.200408-1147OC
Yang, Zhenhua ; Yang, Dong ; Kong, Ying ; Zhang, Lixin ; Marrs, Carl F. ; Foxman, Betsy ; Bates, Joseph H. ; Wilson, Frank ; Cave, M. Donald. / Clinical relevance of Mycobacterium tuberculosis plcD gene mutations. In: American Journal of Respiratory and Critical Care Medicine. 2005 ; Vol. 171, No. 12. pp. 1436-1442.
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