Cloning and characterization of the promoter for the liver isoform of the rat carnitine palmitoyltransferase I (L-CPT I) gene

Edwards Park, Michelle L. Steffen, Shulan Song, Vicki M. Park, George Cook

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Carnitine palmitoyltransferase I(CPT I) catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane. The cDNAs of two isoforms of CPT I, termed the hepatic and muscle isoforms, have been cloned. Expression of the hepatic CPT I gene (L-CPT I) is subject to developmental, hormonal and tissue specific regulation. We have cloned the promoter of the L-CPT I gene from a rat genomic library. In the L-CPT I gene, there are two exons 5' to the exon containing the ATG that initiates translation. Exon 1 and the 5' end of exon 2 contain sequences that were not previously described in the rat L-CPT I cDNA. There is an alternatively spliced form of the L-CPT I mRNA in which exon 2 is skipped. The proximal promoter of the L-CPT I gene is extremely GC rich and does not contain a TATA box. There are several putative Sp1 binding sites near the transcriptional start site. A 190 base pair fragment of the promoter can efficiently drive transcription of luciferase and CAT (chloramphenicol acetyltransferase) reporter genes transiently transfected into HepG2 cells. Sequences in both the first intron and the promoter contribute to basal expression, Our results provide the foundation for further studies into the regulation of L-CPT I gene expression.

Original languageEnglish (US)
Pages (from-to)217-224
Number of pages8
JournalBiochemical Journal
Volume330
Issue number1
DOIs
StatePublished - Feb 15 1998

Fingerprint

Carnitine O-Palmitoyltransferase
Carnitine
Cloning
Liver
Rats
Organism Cloning
Protein Isoforms
Genes
Exons
Complementary DNA
TATA Box
Chloramphenicol O-Acetyltransferase
Genomic Library
Hep G2 Cells
Mitochondrial Membranes
Transcription
Luciferases
Reporter Genes
Gene expression
Base Pairing

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and characterization of the promoter for the liver isoform of the rat carnitine palmitoyltransferase I (L-CPT I) gene. / Park, Edwards; Steffen, Michelle L.; Song, Shulan; Park, Vicki M.; Cook, George.

In: Biochemical Journal, Vol. 330, No. 1, 15.02.1998, p. 217-224.

Research output: Contribution to journalArticle

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