Cloning and expression of pkaC and pkaR, the genes encoding the cAMP-dependent protein kinase of Aspergillus fumigatus

Brain G. Oliver, John C. Panepinto, Jarrod Fortwendel, Darcey L. Smith, David S. Askew, Judith C. Rhodes

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This report describes the cloning and expression of both subunits of PKA in the opportunistic fungal pathogen Aspergillus fumigatus. The predicted translation product of the regulatory subunit, pkaR, is defined as a type II regulatory subunit. The gene encoding the A. fumigatus catalytic subunit, pkaC, contains the conserved kinase and activation domains that are characteristic of PkaC proteins. Both subunit mRNAs are expressed throughout the asexual life cycle of A. fumigatus. Message levels of pkaR and pkaC are higher during co-cultivation with alveolar epithelial cells than during culture alone.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalMycopathologia
Volume154
Issue number2
DOIs
StatePublished - Jun 19 2002
Externally publishedYes

Fingerprint

Aspergillus fumigatus
cAMP-dependent protein kinase
Cyclic AMP-Dependent Protein Kinases
Organism Cloning
molecular cloning
Genes
Alveolar Epithelial Cells
genes
protein subunits
Life Cycle Stages
translation (genetics)
life cycle (organisms)
Catalytic Domain
phosphotransferases (kinases)
epithelial cells
Phosphotransferases
Cell Culture Techniques
Messenger RNA
pathogens
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Agronomy and Crop Science
  • veterinary (miscalleneous)
  • Plant Science

Cite this

Cloning and expression of pkaC and pkaR, the genes encoding the cAMP-dependent protein kinase of Aspergillus fumigatus. / Oliver, Brain G.; Panepinto, John C.; Fortwendel, Jarrod; Smith, Darcey L.; Askew, David S.; Rhodes, Judith C.

In: Mycopathologia, Vol. 154, No. 2, 19.06.2002, p. 85-91.

Research output: Contribution to journalArticle

Oliver, Brain G. ; Panepinto, John C. ; Fortwendel, Jarrod ; Smith, Darcey L. ; Askew, David S. ; Rhodes, Judith C. / Cloning and expression of pkaC and pkaR, the genes encoding the cAMP-dependent protein kinase of Aspergillus fumigatus. In: Mycopathologia. 2002 ; Vol. 154, No. 2. pp. 85-91.
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