Cloning, developmental regulation and neural localization of rat ε-sarcoglycan

Research output: Contribution to journalArticle

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Abstract

Mutations in the gene for epsilon sarcoglycan (ε-SG) are associated with a disorder of the central nervous system, the myoclonus-dystonia syndrome (MDS; DYT11). In contrast, mutations of other sarcoglycan family members lead to limb-girdle muscular dystrophies. To establish the framework for functional studies of ε-SG, we cloned rat ε-SG cDNA, quantified ε-SG mRNA levels in neural and non-neural tissues at different developmental time points with relative quantitative multiplex real-time reverse transcriptase PCR (RT-PCR), and characterized the distribution of ε-SG mRNA in brain with in situ hybridization. Rat ε-SG cDNA contains an open reading frame (ORF) of 1311 bp that encodes a 437-amino acid (aa) protein with 95.9% and 98.2% identity to human and mouse ε-SG amino acid sequences, respectively. Using real-time RT-PCR, ε-SG was detected in both neural (cerebellar cortex, striatum, cerebral cortex, thalamus, hippocampus) and non-neural (muscle, liver, kidney, heart) tissues at each developmental time point tested [Embryonic Day 20 (E20), Postnatal Day 1 (P1), P7, P14, P36, 6 months, 1.5 years). Levels of ε-SG mRNA were highest at E20 in all tissues. The developmental regulation of ε-SG mRNA expression was most striking in muscle with E20 and early postnatal ε-SG mRNA levels over 10 times higher than those seen in adult rats. In adult rats, ε-SG mRNA levels were several-fold higher in brain, particularly cerebellar cortex, than in muscle. Radioactive in situ hybridization showed that ε-SG mRNA was widely distributed in rat brain. Robust hybridization signal was obtained from regions with dense neuronal packing such as the hippocampus, cerebellar molecular layer, and cerebral cortex. Our results suggest that ε-SG participates in the development of both neural and non-neural tissues and contributes to neuronal structure in the adult central nervous system.

Original languageEnglish (US)
Pages (from-to)132-143
Number of pages12
JournalMolecular Brain Research
Volume119
Issue number2
DOIs
StatePublished - Nov 26 2003

Fingerprint

Sarcoglycans
Organism Cloning
Messenger RNA
Cerebellar Cortex
Reverse Transcriptase Polymerase Chain Reaction
Muscles
Cerebral Cortex
In Situ Hybridization
Real-Time Polymerase Chain Reaction
Hippocampus
Brain
Complementary DNA
Limb-Girdle Muscular Dystrophies
Mutation
Central Nervous System Diseases
Thalamus
Open Reading Frames
Amino Acid Sequence
Central Nervous System
Kidney

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Cloning, developmental regulation and neural localization of rat ε-sarcoglycan. / Xiao, Jianfeng; Ledoux, Mark.

In: Molecular Brain Research, Vol. 119, No. 2, 26.11.2003, p. 132-143.

Research output: Contribution to journalArticle

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abstract = "Mutations in the gene for epsilon sarcoglycan (ε-SG) are associated with a disorder of the central nervous system, the myoclonus-dystonia syndrome (MDS; DYT11). In contrast, mutations of other sarcoglycan family members lead to limb-girdle muscular dystrophies. To establish the framework for functional studies of ε-SG, we cloned rat ε-SG cDNA, quantified ε-SG mRNA levels in neural and non-neural tissues at different developmental time points with relative quantitative multiplex real-time reverse transcriptase PCR (RT-PCR), and characterized the distribution of ε-SG mRNA in brain with in situ hybridization. Rat ε-SG cDNA contains an open reading frame (ORF) of 1311 bp that encodes a 437-amino acid (aa) protein with 95.9{\%} and 98.2{\%} identity to human and mouse ε-SG amino acid sequences, respectively. Using real-time RT-PCR, ε-SG was detected in both neural (cerebellar cortex, striatum, cerebral cortex, thalamus, hippocampus) and non-neural (muscle, liver, kidney, heart) tissues at each developmental time point tested [Embryonic Day 20 (E20), Postnatal Day 1 (P1), P7, P14, P36, 6 months, 1.5 years). Levels of ε-SG mRNA were highest at E20 in all tissues. The developmental regulation of ε-SG mRNA expression was most striking in muscle with E20 and early postnatal ε-SG mRNA levels over 10 times higher than those seen in adult rats. In adult rats, ε-SG mRNA levels were several-fold higher in brain, particularly cerebellar cortex, than in muscle. Radioactive in situ hybridization showed that ε-SG mRNA was widely distributed in rat brain. Robust hybridization signal was obtained from regions with dense neuronal packing such as the hippocampus, cerebellar molecular layer, and cerebral cortex. Our results suggest that ε-SG participates in the development of both neural and non-neural tissues and contributes to neuronal structure in the adult central nervous system.",
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