Cloning, expression and characterization of ferret CXCL10

Ali Danesh, Charit Seneviratne, Cheryl M. Cameron, David Banner, Mark E. Devries, Alyson A. Kelvin, Luoling Xu, Longsi Ran, Steven E. Bosinger, Thomas Rowe, Marcus Czub, Colleen Jonsson, Mark J. Cameron, David J. Kelvin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Chemokines and their receptors function in the recruitment and activation of cells of the immune system to sites of inflammation. As such, chemokines play an important role in mediating pathophysiological events during microbial infection. In particular, CXCL9, CXCL10 and CXCL11 and their cognate receptor CXCR3 have been associated with the clinical course of several infectious diseases, including severe acute respiratory syndrome (SARS) and influenza. While CXCL9, CXCL10 and CXCL11 share the same receptor and have overlapping functions, each can also have unique activity in host defense. The lack of a preferred characterized animal model for SARS has brought our attention to ferrets, which have been used for years in influenza studies. The lack of immunological reagents for ferrets prompted us to clone CXCL9, CXCL10, CXCL11 and CXCR3 and, in the case of CXCL10, to express the gene as a recombinant protein. In this study we demonstrate that endogenous ferret CXCL10 exhibits similar mRNA expression patterns in the lungs of deceased SARS patients and ferrets experimentally infected with SARS coronavirus. This study therefore represents an important step towards development of the ferret as a model for the role of CXCL9, CXCL10 and CXCL11:CXCR3 axis in severe viral infections.

Original languageEnglish (US)
Pages (from-to)1288-1297
Number of pages10
JournalMolecular Immunology
Volume45
Issue number5
DOIs
StatePublished - Mar 1 2008

Fingerprint

Ferrets
Severe Acute Respiratory Syndrome
Organism Cloning
Human Influenza
CXCR3 Receptors
Coronavirus
Chemokine Receptors
Virus Diseases
Chemokines
Recombinant Proteins
Communicable Diseases
Immune System
Animal Models
Clone Cells
Inflammation
Lung
Messenger RNA
Infection
Genes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Danesh, A., Seneviratne, C., Cameron, C. M., Banner, D., Devries, M. E., Kelvin, A. A., ... Kelvin, D. J. (2008). Cloning, expression and characterization of ferret CXCL10. Molecular Immunology, 45(5), 1288-1297. https://doi.org/10.1016/j.molimm.2007.09.018

Cloning, expression and characterization of ferret CXCL10. / Danesh, Ali; Seneviratne, Charit; Cameron, Cheryl M.; Banner, David; Devries, Mark E.; Kelvin, Alyson A.; Xu, Luoling; Ran, Longsi; Bosinger, Steven E.; Rowe, Thomas; Czub, Marcus; Jonsson, Colleen; Cameron, Mark J.; Kelvin, David J.

In: Molecular Immunology, Vol. 45, No. 5, 01.03.2008, p. 1288-1297.

Research output: Contribution to journalArticle

Danesh, A, Seneviratne, C, Cameron, CM, Banner, D, Devries, ME, Kelvin, AA, Xu, L, Ran, L, Bosinger, SE, Rowe, T, Czub, M, Jonsson, C, Cameron, MJ & Kelvin, DJ 2008, 'Cloning, expression and characterization of ferret CXCL10', Molecular Immunology, vol. 45, no. 5, pp. 1288-1297. https://doi.org/10.1016/j.molimm.2007.09.018
Danesh A, Seneviratne C, Cameron CM, Banner D, Devries ME, Kelvin AA et al. Cloning, expression and characterization of ferret CXCL10. Molecular Immunology. 2008 Mar 1;45(5):1288-1297. https://doi.org/10.1016/j.molimm.2007.09.018
Danesh, Ali ; Seneviratne, Charit ; Cameron, Cheryl M. ; Banner, David ; Devries, Mark E. ; Kelvin, Alyson A. ; Xu, Luoling ; Ran, Longsi ; Bosinger, Steven E. ; Rowe, Thomas ; Czub, Marcus ; Jonsson, Colleen ; Cameron, Mark J. ; Kelvin, David J. / Cloning, expression and characterization of ferret CXCL10. In: Molecular Immunology. 2008 ; Vol. 45, No. 5. pp. 1288-1297.
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