Cloning of a bovine orphan transporter and its short splicing variant

Kazuko Sakata, Shoichi Shimada, Toshihide Yamashita, Kiyoshi Inoue, Masaya Tohyama

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have isolated a cDNA (bv7-3) encoding a member of the Na+,Cl--dependent transporter family and its short splicing variant (bv7-3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7-3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7-3-2. bv7-3s contains 265 amino acids, sharing 252 N-terminal amino acids with bv7-3. Both mRNAs for bv7-3 and bv7-3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc-tagged bv7-3 was mainly detected on the plasma membrane, whereas myc-tagged bv7-3s showed a pattern of intracellular membrane staining. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)267-270
Number of pages4
JournalFEBS Letters
Volume443
Issue number3
DOIs
StatePublished - Jan 29 1999
Externally publishedYes

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Cloning
Organism Cloning
Amino Acids
Intracellular Membranes
HEK293 Cells
Neurology
Cell membranes
Gene Library
Northern Blotting
Nervous System
Fluorescent Antibody Technique
Sequence Analysis
Retina
Rats
Screening
Complementary DNA
Cell Membrane
Staining and Labeling
Membranes
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cloning of a bovine orphan transporter and its short splicing variant. / Sakata, Kazuko; Shimada, Shoichi; Yamashita, Toshihide; Inoue, Kiyoshi; Tohyama, Masaya.

In: FEBS Letters, Vol. 443, No. 3, 29.01.1999, p. 267-270.

Research output: Contribution to journalArticle

Sakata, K, Shimada, S, Yamashita, T, Inoue, K & Tohyama, M 1999, 'Cloning of a bovine orphan transporter and its short splicing variant', FEBS Letters, vol. 443, no. 3, pp. 267-270. https://doi.org/10.1016/S0014-5793(98)01733-5
Sakata, Kazuko ; Shimada, Shoichi ; Yamashita, Toshihide ; Inoue, Kiyoshi ; Tohyama, Masaya. / Cloning of a bovine orphan transporter and its short splicing variant. In: FEBS Letters. 1999 ; Vol. 443, No. 3. pp. 267-270.
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