Cloning of a lymphatic peptide/histidine transporter

Kazuko Sakata, Toshihide Yamashita, Mitsuyo Maeda, Yoshinori Moriyama, Shoichi Shimada, Masaya Tohyama

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Although peptide transport across the plasma membrane has been characterized well in the kidney and the intestine, the functional relevance of this transport in other organs has not been addressed. Here we report the cloning of a cDNA for a novel peptide/histidine transporter found in the rat (rPHT2), whose mRNA is expressed mainly in the lymphatic system. rPHT2 encodes a protein of 582 amino acids and showed 49% identity with the brain PHT (PHT1) [Yamashita, Shimada, Guo, Sato, Kohmura, Hayakawa, Takagi and Tohyama (1997) J. Biol. Chem. 272, 10205-10211]. rPHT2 mRNA was abundant in lung, spleen and thymus, and detected faintly in brain, liver, adrenal gland and heart by Northern-blot analysis and reverse transcriptase PCR (RT-PCR). Intense signals for the gene were found in immunocytes using in situ hybridization. Ectopic expression of rPHT2 protein in HEK-293T cells and BHK cells was not found on the cell surface, but was found on the lysosomal membrane using light- and electron-microscopic analysis Recombinant rPHT2 protein reconstituted into liposomes showed proton-dependent transport activity with histidine and histidyl-leucine. These findings suggest that rPHT2 is involved in the protein catabolic pathway in the lymphatic system.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalBiochemical Journal
Volume356
Issue number1
DOIs
StatePublished - May 15 2001

Fingerprint

Cloning
Histidine
Organism Cloning
Lymphatic System
Peptides
Brain
Thymus
Messenger RNA
Proteins
HEK293 Cells
RNA-Directed DNA Polymerase
Cell membranes
Adrenal Glands
Reverse Transcriptase Polymerase Chain Reaction
Recombinant Proteins
Liposomes
Leucine
Northern Blotting
Liver
Thymus Gland

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sakata, K., Yamashita, T., Maeda, M., Moriyama, Y., Shimada, S., & Tohyama, M. (2001). Cloning of a lymphatic peptide/histidine transporter. Biochemical Journal, 356(1), 53-60. https://doi.org/10.1042/0264-6021:3560053

Cloning of a lymphatic peptide/histidine transporter. / Sakata, Kazuko; Yamashita, Toshihide; Maeda, Mitsuyo; Moriyama, Yoshinori; Shimada, Shoichi; Tohyama, Masaya.

In: Biochemical Journal, Vol. 356, No. 1, 15.05.2001, p. 53-60.

Research output: Contribution to journalArticle

Sakata, K, Yamashita, T, Maeda, M, Moriyama, Y, Shimada, S & Tohyama, M 2001, 'Cloning of a lymphatic peptide/histidine transporter', Biochemical Journal, vol. 356, no. 1, pp. 53-60. https://doi.org/10.1042/0264-6021:3560053
Sakata K, Yamashita T, Maeda M, Moriyama Y, Shimada S, Tohyama M. Cloning of a lymphatic peptide/histidine transporter. Biochemical Journal. 2001 May 15;356(1):53-60. https://doi.org/10.1042/0264-6021:3560053
Sakata, Kazuko ; Yamashita, Toshihide ; Maeda, Mitsuyo ; Moriyama, Yoshinori ; Shimada, Shoichi ; Tohyama, Masaya. / Cloning of a lymphatic peptide/histidine transporter. In: Biochemical Journal. 2001 ; Vol. 356, No. 1. pp. 53-60.
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