Cloning of acyl-acyl carrier protein (ACP) thioesterase gene from Brassica juncea

Nawajes Mandal, I. M. Santha, M. L. Lodha, S. L. Mehta

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Acyl-acyl carrier protein (ACP) thioesterase is the chain-length-determining enzyme in de novo biosynthesis of plant fatty acids. For cloning the gene encoding acyl-ACP thioesterase from Brassica juncea, genomic DNA was used as a template to amplify a 0.7 kb thioesterase fragment in a PCR with the primers designed from the known sequences available in the GenBank. This 0.7 kb fragment was used as a probe to study the expression of the gene in developing seeds and also to screen a genomic library of B. juncea constructed in λEMBL-3 to get the full length of the gene. A 4.0 kb BamHI fragment containing the full gene was finally cloned in a plasmid vector from a recombinant phage clone λ5.12 after a series of screening, sub-cloning and Southern hybridization.

Original languageEnglish (US)
Pages (from-to)967-969
Number of pages3
JournalBiochemical Society Transactions
Volume28
Issue number6
DOIs
StatePublished - Jan 1 2000

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Mustard Plant
Cloning
Organism Cloning
Genes
Bacteriophages
Gene encoding
Genomic Library
Biosynthesis
Nucleic Acid Databases
Chain length
Seed
Seeds
Screening
Plasmids
Fatty Acids
Clone Cells
Gene Expression
Polymerase Chain Reaction
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Cloning of acyl-acyl carrier protein (ACP) thioesterase gene from Brassica juncea. / Mandal, Nawajes; Santha, I. M.; Lodha, M. L.; Mehta, S. L.

In: Biochemical Society Transactions, Vol. 28, No. 6, 01.01.2000, p. 967-969.

Research output: Contribution to journalArticle

Mandal, Nawajes ; Santha, I. M. ; Lodha, M. L. ; Mehta, S. L. / Cloning of acyl-acyl carrier protein (ACP) thioesterase gene from Brassica juncea. In: Biochemical Society Transactions. 2000 ; Vol. 28, No. 6. pp. 967-969.
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