Cloning of human myosin-X

An unconventional myosin

T. J. O'Farrell, J. T. Anderson, Tayebeh Pourmotabbed

Research output: Contribution to journalArticle

Abstract

Myosin-X is a new member of the unconventional myosin superfamily. The Frog and bovine myosin-X have been identified and sequenced recently by Sole et. al. (1994) and Cori et. al. (1996), respectively. We have cloned a partial eDNA for the human counterpart of this protein from a chronic granulocytic leukemia eDNA library. The partial eDNA encodes for the carboxy terminal 548 amino acids of tail of the myosin-X. This includes the MYTH4 domain, and the talin-homology domain with 93% amino acid sequence identity to the bovine protein. Myosin-X appears to be widely distributed in human tissues. It's mRNA is particularly abundant in kidney, brain (putamen and Caudate nucleus), thyroid and pituitary glands, and testis. Although, the biological role of myosin-X is unclear, on the basis of its amino acid similarities to a large number of proteins that interact with the membrane cytoskeleton and signal transduction machinery, it has been proposed to play a role linking the signal transduction pathway to the actin cytoskeleton.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - Dec 1 1997

Fingerprint

Cloning
Myosins
Organism Cloning
Signal transduction
Amino Acids
Signal Transduction
Talin
Proteins
Caudate Nucleus
Putamen
Pituitary Gland
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Cytoskeleton
Actin Cytoskeleton
Anura
Libraries
Machinery
Tail
Testis
Actins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Cloning of human myosin-X : An unconventional myosin. / O'Farrell, T. J.; Anderson, J. T.; Pourmotabbed, Tayebeh.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997.

Research output: Contribution to journalArticle

O'Farrell, TJ, Anderson, JT & Pourmotabbed, T 1997, 'Cloning of human myosin-X: An unconventional myosin', FASEB Journal, vol. 11, no. 9.
O'Farrell, T. J. ; Anderson, J. T. ; Pourmotabbed, Tayebeh. / Cloning of human myosin-X : An unconventional myosin. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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