CM1, a possible novel activation molecule on human lymphocytes

Dae Y. Hur, Seonghan Kim, Young In Kim Hoehamer, Hye Y. Min, Dae J. Kim, Dong Sup Lee, Daeho Cho, Young Il Hwang, Douk H. Hwang, Seong H. Park, Hee K. Ahn, Ka Y. Chang, Yoon B. Kim, Wang J. Lee

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Abstract

CM1 (centrocyte/-blast marker 1) defined by a mAb developed against concanavalin-A activated PBMC, is expressed specifically on some tonsillar germinal center (GC) B cells. In single flow cytometric analysis, the bone marrow did not express these molecules nor did the PBMC or the thymocytes. The peripheral B lymphocytes showed more than 90% positive, while the peripheral T lymphocytes showed approximately 60% positive at 48 h after activation by PMA/ionomycin, respectively. A western blot analysis and an immunoprecipitation for CM1 showed a band at 70 kDa. Cross-linking of CM1 with anti-CM1 mAb induced apoptosis of the GC B cells (CD38+IgD-). Immunohistochemical staining revealed that the CM1 molecule is distributed over the entire area except the proximal dark zone of the tonsillar germinal centers. These results suggest that the CM1 molecule might be involved in differentiation of the germinal center B cells as one of the novel centrocyte markers. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)95-102
Number of pages8
JournalImmunology Letters
Volume74
Issue number2
DOIs
StatePublished - Oct 3 2000

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Germinal Center
B-Lymphocytes
Lymphocytes
Immunoglobulin D
Ionomycin
Thymocytes
Concanavalin A
Immunoprecipitation
Bone Marrow
Western Blotting
Apoptosis
Staining and Labeling
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Hur, D. Y., Kim, S., Kim Hoehamer, Y. I., Min, H. Y., Kim, D. J., Lee, D. S., ... Lee, W. J. (2000). CM1, a possible novel activation molecule on human lymphocytes. Immunology Letters, 74(2), 95-102. https://doi.org/10.1016/S0165-2478(00)00201-7

CM1, a possible novel activation molecule on human lymphocytes. / Hur, Dae Y.; Kim, Seonghan; Kim Hoehamer, Young In; Min, Hye Y.; Kim, Dae J.; Lee, Dong Sup; Cho, Daeho; Hwang, Young Il; Hwang, Douk H.; Park, Seong H.; Ahn, Hee K.; Chang, Ka Y.; Kim, Yoon B.; Lee, Wang J.

In: Immunology Letters, Vol. 74, No. 2, 03.10.2000, p. 95-102.

Research output: Contribution to journalArticle

Hur, DY, Kim, S, Kim Hoehamer, YI, Min, HY, Kim, DJ, Lee, DS, Cho, D, Hwang, YI, Hwang, DH, Park, SH, Ahn, HK, Chang, KY, Kim, YB & Lee, WJ 2000, 'CM1, a possible novel activation molecule on human lymphocytes', Immunology Letters, vol. 74, no. 2, pp. 95-102. https://doi.org/10.1016/S0165-2478(00)00201-7
Hur DY, Kim S, Kim Hoehamer YI, Min HY, Kim DJ, Lee DS et al. CM1, a possible novel activation molecule on human lymphocytes. Immunology Letters. 2000 Oct 3;74(2):95-102. https://doi.org/10.1016/S0165-2478(00)00201-7
Hur, Dae Y. ; Kim, Seonghan ; Kim Hoehamer, Young In ; Min, Hye Y. ; Kim, Dae J. ; Lee, Dong Sup ; Cho, Daeho ; Hwang, Young Il ; Hwang, Douk H. ; Park, Seong H. ; Ahn, Hee K. ; Chang, Ka Y. ; Kim, Yoon B. ; Lee, Wang J. / CM1, a possible novel activation molecule on human lymphocytes. In: Immunology Letters. 2000 ; Vol. 74, No. 2. pp. 95-102.
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AB - CM1 (centrocyte/-blast marker 1) defined by a mAb developed against concanavalin-A activated PBMC, is expressed specifically on some tonsillar germinal center (GC) B cells. In single flow cytometric analysis, the bone marrow did not express these molecules nor did the PBMC or the thymocytes. The peripheral B lymphocytes showed more than 90% positive, while the peripheral T lymphocytes showed approximately 60% positive at 48 h after activation by PMA/ionomycin, respectively. A western blot analysis and an immunoprecipitation for CM1 showed a band at 70 kDa. Cross-linking of CM1 with anti-CM1 mAb induced apoptosis of the GC B cells (CD38+IgD-). Immunohistochemical staining revealed that the CM1 molecule is distributed over the entire area except the proximal dark zone of the tonsillar germinal centers. These results suggest that the CM1 molecule might be involved in differentiation of the germinal center B cells as one of the novel centrocyte markers. (C) 2000 Elsevier Science B.V.

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