CNP-1 (Arrd-17), a novel substrate of calcineurin, is critical for modulation of egg-laying and locomotion in response to food and lysine sensation in Caenorhabditis elegans

Chang Hoon Jee, Tae Woo Choi, Karunambigai Kalichamy, Jong Zin Yee, Hyun Ok Song, Yon Ju Ji, Jungsoo Lee, Jin Il Lee, Noelle D. L'Etoile, Joohong Ahnn, Sun Kyung Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Calcineurin is a Ca 2+/calmodulin-dependent protein phosphatase involved in calcium signaling pathways. In Caenorhabditis elegans, the loss of calcineurin activity causes pleiotropic defects including hyperadaptation of sensory neurons, hypersensation to thermal difference and hyper-egg-laying when worms are refed after starvation. In this study, we report on arrd-17 as calcineurin-interacting protein-1 (cnp-1), which is a novel molecular target of calcineurin. CNP-1 interacts with the catalytic domain of the C. elegans calcineurin A subunit, TAX-6, in a yeast two-hybrid assay and is dephosphorylated by TAX-6 in vitro. cnp-1 is expressed in ASK, ADL, ASH and ASJ sensory neurons as TAX-6. It acts downstream of tax-6 in regulation of locomotion and egg-laying after starvation, ASH sensory neuron adaptation and lysine chemotaxis, that is known to be mediated by ASK neurons. Altogether, our biochemical and genetic evidence indicates that CNP-1 is a direct target of calcineurin and required in stimulated egg-laying and locomotion after starvation, adaptation to hyperosmolarity and attraction to lysine, which is modulated by calcineurin. We suggest that the phosphorylation status of CNP-1 plays an important role in regulation of refed stimulating behaviors after starvation and attraction to amino acid, which provides valuable nutritious information.

Original languageEnglish (US)
Pages (from-to)165-178
Number of pages14
JournalJournal of Molecular Biology
Volume417
Issue number3
DOIs
StatePublished - Mar 30 2012
Externally publishedYes

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Calcineurin
Caenorhabditis elegans
Locomotion
Lysine
Ovum
Food
Starvation
Sensory Receptor Cells
Two-Hybrid System Techniques
Calcium Signaling
Phosphoprotein Phosphatases
Chemotaxis
Calmodulin
Activities of Daily Living
Molecular Biology
Catalytic Domain
Proteins
Hot Temperature
Phosphorylation
Neurons

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

CNP-1 (Arrd-17), a novel substrate of calcineurin, is critical for modulation of egg-laying and locomotion in response to food and lysine sensation in Caenorhabditis elegans. / Jee, Chang Hoon; Choi, Tae Woo; Kalichamy, Karunambigai; Yee, Jong Zin; Song, Hyun Ok; Ji, Yon Ju; Lee, Jungsoo; Lee, Jin Il; L'Etoile, Noelle D.; Ahnn, Joohong; Lee, Sun Kyung.

In: Journal of Molecular Biology, Vol. 417, No. 3, 30.03.2012, p. 165-178.

Research output: Contribution to journalArticle

Jee, Chang Hoon ; Choi, Tae Woo ; Kalichamy, Karunambigai ; Yee, Jong Zin ; Song, Hyun Ok ; Ji, Yon Ju ; Lee, Jungsoo ; Lee, Jin Il ; L'Etoile, Noelle D. ; Ahnn, Joohong ; Lee, Sun Kyung. / CNP-1 (Arrd-17), a novel substrate of calcineurin, is critical for modulation of egg-laying and locomotion in response to food and lysine sensation in Caenorhabditis elegans. In: Journal of Molecular Biology. 2012 ; Vol. 417, No. 3. pp. 165-178.
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AU - Jee, Chang Hoon

AU - Choi, Tae Woo

AU - Kalichamy, Karunambigai

AU - Yee, Jong Zin

AU - Song, Hyun Ok

AU - Ji, Yon Ju

AU - Lee, Jungsoo

AU - Lee, Jin Il

AU - L'Etoile, Noelle D.

AU - Ahnn, Joohong

AU - Lee, Sun Kyung

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AB - Calcineurin is a Ca 2+/calmodulin-dependent protein phosphatase involved in calcium signaling pathways. In Caenorhabditis elegans, the loss of calcineurin activity causes pleiotropic defects including hyperadaptation of sensory neurons, hypersensation to thermal difference and hyper-egg-laying when worms are refed after starvation. In this study, we report on arrd-17 as calcineurin-interacting protein-1 (cnp-1), which is a novel molecular target of calcineurin. CNP-1 interacts with the catalytic domain of the C. elegans calcineurin A subunit, TAX-6, in a yeast two-hybrid assay and is dephosphorylated by TAX-6 in vitro. cnp-1 is expressed in ASK, ADL, ASH and ASJ sensory neurons as TAX-6. It acts downstream of tax-6 in regulation of locomotion and egg-laying after starvation, ASH sensory neuron adaptation and lysine chemotaxis, that is known to be mediated by ASK neurons. Altogether, our biochemical and genetic evidence indicates that CNP-1 is a direct target of calcineurin and required in stimulated egg-laying and locomotion after starvation, adaptation to hyperosmolarity and attraction to lysine, which is modulated by calcineurin. We suggest that the phosphorylation status of CNP-1 plays an important role in regulation of refed stimulating behaviors after starvation and attraction to amino acid, which provides valuable nutritious information.

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