Coculture of THP-1 human mononuclear cells with Candida albicans results in pronounced changes in host gene expression

Katherine S. Barker, Teresa Liu, Phillip Rogers

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background. The host's first line of defense against bloodstream infection with Candida albicans involves the recognition and clearance of the fungus by neutrophils and monocytes/macrophages. The purpose of the present study was to examine changes in the monocytic cell gene-expression profile in response to C. albicans stimulation. Methods. RNA was isolated from THP-1 cells 3 h after coculture with live C. albicans SC5314 cells. After hybridization to microarrays, genes differentially expressed by at least 2.0-fold were included in the final data set. Results. As expected, TNFA, IL8, CD83, MIPlA, and MIP1B were among the genes up-regulated. This was confirmed by real-time reverse-transcriptase polymerase chain reaction (RT-PCR), fluorescence-activated cell sorting analysis, and enzyme-linked immunosorbent assay. Furthermore, RGS1, RGS2, RGS16, DSCR1, GROB, EGR3, FLT4, and TNFAIP6 were also up-regulated in response to C. albicans, whereas CCR2 and NCF2 were among the genes down-regulated in response to C. albicans. Differential expression of selected genes was confirmed at several time points by real-time RT-PCR. Conclusions. This study defines the gene expression profile of an early response of human mononuclear cells to C. albicans and identifies genes not previously known to be responsive to this pathogen.

Original languageEnglish (US)
Pages (from-to)901-912
Number of pages12
JournalJournal of Infectious Diseases
Volume192
Issue number5
DOIs
StatePublished - Sep 1 2005

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Coculture Techniques
Candida albicans
Gene Expression
Reverse Transcriptase Polymerase Chain Reaction
Transcriptome
Genes
Real-Time Polymerase Chain Reaction
Interleukin-8
Monocytes
Flow Cytometry
Neutrophils
Fungi
Enzyme-Linked Immunosorbent Assay
Macrophages
RNA
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Coculture of THP-1 human mononuclear cells with Candida albicans results in pronounced changes in host gene expression. / Barker, Katherine S.; Liu, Teresa; Rogers, Phillip.

In: Journal of Infectious Diseases, Vol. 192, No. 5, 01.09.2005, p. 901-912.

Research output: Contribution to journalArticle

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abstract = "Background. The host's first line of defense against bloodstream infection with Candida albicans involves the recognition and clearance of the fungus by neutrophils and monocytes/macrophages. The purpose of the present study was to examine changes in the monocytic cell gene-expression profile in response to C. albicans stimulation. Methods. RNA was isolated from THP-1 cells 3 h after coculture with live C. albicans SC5314 cells. After hybridization to microarrays, genes differentially expressed by at least 2.0-fold were included in the final data set. Results. As expected, TNFA, IL8, CD83, MIPlA, and MIP1B were among the genes up-regulated. This was confirmed by real-time reverse-transcriptase polymerase chain reaction (RT-PCR), fluorescence-activated cell sorting analysis, and enzyme-linked immunosorbent assay. Furthermore, RGS1, RGS2, RGS16, DSCR1, GROB, EGR3, FLT4, and TNFAIP6 were also up-regulated in response to C. albicans, whereas CCR2 and NCF2 were among the genes down-regulated in response to C. albicans. Differential expression of selected genes was confirmed at several time points by real-time RT-PCR. Conclusions. This study defines the gene expression profile of an early response of human mononuclear cells to C. albicans and identifies genes not previously known to be responsive to this pathogen.",
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