Collagen heterogeneity in the avian eye

Lens, vitreous body, cornea and sclera

Robert L. Trelstad, Andrew Kang

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In order to further investigate the molecular heterogeneity of the primary structure of collagen, we have extracted, purified and characterized collagens from the various component tissues in the eyes of lathyritic and normal chickens. The eye tissues were dissected arbitrarily into four portions; the cornea, the lens, the vitreous body and the sclera. The collagen isolated from the cornea as well as the fibrous portion of the sclera was shown to consist of [α1(I)]2α2 by the criteria of CM-cellulose chromatography, and amino acid analysis. The collagens isolated from the lens and the vitreous body contained predominantly α1 chains whose amino acid composition differs significantly from that previously obtained for chick α1(I), α1(II) or α2. Pepsin-solubilization of the cartilaginous portion of the sclera and subsequent CM-cellulose chromatography revealed, in addition to the anticipated α1(II), another fraction eluting after α2. Thus, we conclude that the avian eye contains several distinct species of collagens. In addition to the [α1(I)]2α2 and [α1(II)]3 in the cornea, fibrous sclera and cartilaginous sclera respectively, our data indicate the presence in the lens and the vitreous body of a species of collagen composed of α1-chains previously not described and in the scleral cartilage an α2-like molecule with an unusual amino acid composition.

Original languageEnglish (US)
Pages (from-to)395-406
Number of pages12
JournalExperimental Eye Research
Volume18
Issue number4
DOIs
StatePublished - Jan 1 1974

Fingerprint

Vitreous Body
Crystalline Lens
Sclera
Cornea
Collagen
Lenses
Amino Acids
Cellulose
Chromatography
Pepsin A
Cartilage
Chickens

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Collagen heterogeneity in the avian eye : Lens, vitreous body, cornea and sclera. / Trelstad, Robert L.; Kang, Andrew.

In: Experimental Eye Research, Vol. 18, No. 4, 01.01.1974, p. 395-406.

Research output: Contribution to journalArticle

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