Collagen-induced arthritis.

E. F. Rosloniec, M. Cremer, Andrew Kang, Linda Myers

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Collagen-induced arthritis (CIA) is an experimental autoimmune disease that can be elicited in susceptible strains of rodents (rat and mouse) and nonhuman primates by immunization with type II collagen (CII), the major constituent protein of articular cartilage. Because of the important similarities between CIA and rheumatoid arthritis, this experimental model of autoimmune arthritis has been the subject of extensive investigation in several laboratories. Protocols for CIA are described in this unit for both the mouse model and the rat model. In addition, protocols are included for the purification of CII from bovine articular joints and chicken sternums, for the purification of collagen a1(II) chains, and for the purification of fragments of these chains following cyanogen bromide (CNBr) digestion. The preparation of CII is a time-consuming procedure but is usually required because of the scarcity and expense of commercial sources of purified native CII. In addition, support protocols are provided for assessing the severity of inflammation following CIA and for measuring B and T cell responses to CII.

Original languageEnglish (US)
JournalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]
VolumeChapter 15
StatePublished - Jan 1 2001

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Experimental Arthritis
Joints
Cyanogen Bromide
Sternum
Collagen Type II
Articular Cartilage
Primates
Autoimmune Diseases
Arthritis
Digestion
Chickens
Rodentia
Immunization
Rheumatoid Arthritis
B-Lymphocytes
Theoretical Models
Collagen
Inflammation
T-Lymphocytes
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology

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Collagen-induced arthritis. / Rosloniec, E. F.; Cremer, M.; Kang, Andrew; Myers, Linda.

In: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 15, 01.01.2001.

Research output: Contribution to journalArticle

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