Collagen-platelet interaction. Type V (A-B) collagen induces platelet aggregation

T. M. Chiang, C. L. Mainardi, J. M. Seyer, Andrew Kang

Research output: Contribution to journalArticle

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Abstract

One of the more recently discovered collagens, type V (or A-B) collagen, in its native fibrillar form mediates human platelet aggregation and the release of serotonin. In agreement with a recent report, it has no detectable effect on human platelets in the soluble or amorphous form. The possibility that the observed results might be due to contaminating interstitial collagens was eliminated by taking advantage of unusual solubility properties of type V collagen. Type V collagen dissolved in 0.1M acetic acid formed native-type fibrils when dialyzed against PBS and amorphous fibrils when dialyzed against 0.05M Tris/0.13M NaCl, pH 7.4, at 4 °C. Interstitial collagens remained in solution under both of these conditions. In addition, type V collagen treated with sufficient, purified synovial collagenase to digest all contaminating interstitial collagen retained its platelet-aggregating properties. The purity of type V collagen was confirmed by SDS-PAGE of CNBr digests. These data indicate that the quaternary structure of type V collagen is important in its recognition by platelet membranes.

Original languageEnglish (US)
Pages (from-to)99-107
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Volume95
Issue number1
StatePublished - Jan 1 1980

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Collagen Type V
Platelets
Platelet Aggregation
Collagen
Blood Platelets
Agglomeration
Collagenases
Acetic Acid
Solubility
Polyacrylamide Gel Electrophoresis
Serotonin
Membranes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Collagen-platelet interaction. Type V (A-B) collagen induces platelet aggregation. / Chiang, T. M.; Mainardi, C. L.; Seyer, J. M.; Kang, Andrew.

In: The Journal of Laboratory and Clinical Medicine, Vol. 95, No. 1, 01.01.1980, p. 99-107.

Research output: Contribution to journalArticle

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