Collagen synthesis by regenerating rabbit ear tissue

Gerald N. Smith, Andrew Kang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The principal collagen types synthesized during two distinct phases of regeneration in rabbit ears have been investigated, in order to relate altered phenotypic expression in connective tissue cells to regeneration of cartilage. To do this, radioactively labeled collagens synthesized in short-term culture by selected regenerating ear tissues were analyzed by ion-exchange chromatography and SDS-gel electrophoresis of the intact collagens and of the cyanogen bromide peptides derived from them. Prior to the appearance of cartilage, rabbit ear holes are filled by an outgrowth of mesenchyme-like cells derived locally from adjacent tissues. These cells produce a mixture of collagens including type I, [α1(I)]2α2, and the type I trimer, [α1(I)]3, but not type II collagen. Trimer production represents about one-fourth of the collagen synthesized in either a 4-, 10-, or a 24-hr incubation. Trimer is not made by tissues from healing skin wounds nor is it present in normal, uninjured ear tissues. Type II collagen synthesis was detected in tissues taken from late regenerates containing histologically recognizable cartilage, and direct analysis of regenerated cartilage confirmed the presence of type II collagen in the matrix. Thus, regenerated cartilage in the rabbit ear system contains the normal cartilage collagen, type II, while the proliferating cell mass from which the cartilage develops synthesizes the unusual collagen, [α1(I)]3.

Original languageEnglish (US)
Pages (from-to)294-302
Number of pages9
JournalDevelopmental Biology
Volume86
Issue number2
DOIs
StatePublished - Jan 1 1981

Fingerprint

Collagen Type II
Cartilage
Ear
Collagen
Rabbits
Regeneration
Ear Cartilage
Connective Tissue Cells
Cyanogen Bromide
Ion Exchange Chromatography
Mesoderm
Collagen Type I
Wound Healing
Electrophoresis
Gels
Skin
Peptides

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Collagen synthesis by regenerating rabbit ear tissue. / Smith, Gerald N.; Kang, Andrew.

In: Developmental Biology, Vol. 86, No. 2, 01.01.1981, p. 294-302.

Research output: Contribution to journalArticle

Smith, Gerald N. ; Kang, Andrew. / Collagen synthesis by regenerating rabbit ear tissue. In: Developmental Biology. 1981 ; Vol. 86, No. 2. pp. 294-302.
@article{e416388e773c44bc8273b7a34dd7fc85,
title = "Collagen synthesis by regenerating rabbit ear tissue",
abstract = "The principal collagen types synthesized during two distinct phases of regeneration in rabbit ears have been investigated, in order to relate altered phenotypic expression in connective tissue cells to regeneration of cartilage. To do this, radioactively labeled collagens synthesized in short-term culture by selected regenerating ear tissues were analyzed by ion-exchange chromatography and SDS-gel electrophoresis of the intact collagens and of the cyanogen bromide peptides derived from them. Prior to the appearance of cartilage, rabbit ear holes are filled by an outgrowth of mesenchyme-like cells derived locally from adjacent tissues. These cells produce a mixture of collagens including type I, [α1(I)]2α2, and the type I trimer, [α1(I)]3, but not type II collagen. Trimer production represents about one-fourth of the collagen synthesized in either a 4-, 10-, or a 24-hr incubation. Trimer is not made by tissues from healing skin wounds nor is it present in normal, uninjured ear tissues. Type II collagen synthesis was detected in tissues taken from late regenerates containing histologically recognizable cartilage, and direct analysis of regenerated cartilage confirmed the presence of type II collagen in the matrix. Thus, regenerated cartilage in the rabbit ear system contains the normal cartilage collagen, type II, while the proliferating cell mass from which the cartilage develops synthesizes the unusual collagen, [α1(I)]3.",
author = "Smith, {Gerald N.} and Andrew Kang",
year = "1981",
month = "1",
day = "1",
doi = "10.1016/0012-1606(81)90187-1",
language = "English (US)",
volume = "86",
pages = "294--302",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Collagen synthesis by regenerating rabbit ear tissue

AU - Smith, Gerald N.

AU - Kang, Andrew

PY - 1981/1/1

Y1 - 1981/1/1

N2 - The principal collagen types synthesized during two distinct phases of regeneration in rabbit ears have been investigated, in order to relate altered phenotypic expression in connective tissue cells to regeneration of cartilage. To do this, radioactively labeled collagens synthesized in short-term culture by selected regenerating ear tissues were analyzed by ion-exchange chromatography and SDS-gel electrophoresis of the intact collagens and of the cyanogen bromide peptides derived from them. Prior to the appearance of cartilage, rabbit ear holes are filled by an outgrowth of mesenchyme-like cells derived locally from adjacent tissues. These cells produce a mixture of collagens including type I, [α1(I)]2α2, and the type I trimer, [α1(I)]3, but not type II collagen. Trimer production represents about one-fourth of the collagen synthesized in either a 4-, 10-, or a 24-hr incubation. Trimer is not made by tissues from healing skin wounds nor is it present in normal, uninjured ear tissues. Type II collagen synthesis was detected in tissues taken from late regenerates containing histologically recognizable cartilage, and direct analysis of regenerated cartilage confirmed the presence of type II collagen in the matrix. Thus, regenerated cartilage in the rabbit ear system contains the normal cartilage collagen, type II, while the proliferating cell mass from which the cartilage develops synthesizes the unusual collagen, [α1(I)]3.

AB - The principal collagen types synthesized during two distinct phases of regeneration in rabbit ears have been investigated, in order to relate altered phenotypic expression in connective tissue cells to regeneration of cartilage. To do this, radioactively labeled collagens synthesized in short-term culture by selected regenerating ear tissues were analyzed by ion-exchange chromatography and SDS-gel electrophoresis of the intact collagens and of the cyanogen bromide peptides derived from them. Prior to the appearance of cartilage, rabbit ear holes are filled by an outgrowth of mesenchyme-like cells derived locally from adjacent tissues. These cells produce a mixture of collagens including type I, [α1(I)]2α2, and the type I trimer, [α1(I)]3, but not type II collagen. Trimer production represents about one-fourth of the collagen synthesized in either a 4-, 10-, or a 24-hr incubation. Trimer is not made by tissues from healing skin wounds nor is it present in normal, uninjured ear tissues. Type II collagen synthesis was detected in tissues taken from late regenerates containing histologically recognizable cartilage, and direct analysis of regenerated cartilage confirmed the presence of type II collagen in the matrix. Thus, regenerated cartilage in the rabbit ear system contains the normal cartilage collagen, type II, while the proliferating cell mass from which the cartilage develops synthesizes the unusual collagen, [α1(I)]3.

UR - http://www.scopus.com/inward/record.url?scp=0019614396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019614396&partnerID=8YFLogxK

U2 - 10.1016/0012-1606(81)90187-1

DO - 10.1016/0012-1606(81)90187-1

M3 - Article

VL - 86

SP - 294

EP - 302

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -