Collagenaceous, thiol-containing proteins of cnidarian nematocysts

A comparison of the chemistry and protein distribution patterns in two types of cnidae

David Brand, Richard S. Blanquet, Michael A. Phelan

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

1. 1. Nematocyst structural proteins (NSP) from the sea anemones Aiptasia pallida and Metridium senile and the siphonophore Physalia physalis are primarily low molecular weight collagens linked by disulfide bonds. 2. 2. NSP patterns resolved by SDS-PAGE revealed a common, major collagen species (40 kDa) in each nematocyst type, together with other collagens and non-thiol-containing proteins. 3. 3. For each cnidarian, NSP glycosylation profiles were significantly different. 4. 4. Monoclonal antibodies against Aiptasia NSP demonstrated a differential distribution between capsule wall and thread. 5. 5. NSP differences would account for the diversity of morphologic and functional types.

Original languageEnglish (US)
Pages (from-to)115-124
Number of pages10
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume106
Issue number1
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Nematocyst
Cnidaria
Sulfhydryl Compounds
Proteins
Collagen
Hydrozoa
Sea Anemones
Glycosylation
Disulfides
Capsules
Polyacrylamide Gel Electrophoresis
Molecular Weight
Molecular weight
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Molecular Biology

Cite this

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T1 - Collagenaceous, thiol-containing proteins of cnidarian nematocysts

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AU - Phelan, Michael A.

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N2 - 1. 1. Nematocyst structural proteins (NSP) from the sea anemones Aiptasia pallida and Metridium senile and the siphonophore Physalia physalis are primarily low molecular weight collagens linked by disulfide bonds. 2. 2. NSP patterns resolved by SDS-PAGE revealed a common, major collagen species (40 kDa) in each nematocyst type, together with other collagens and non-thiol-containing proteins. 3. 3. For each cnidarian, NSP glycosylation profiles were significantly different. 4. 4. Monoclonal antibodies against Aiptasia NSP demonstrated a differential distribution between capsule wall and thread. 5. 5. NSP differences would account for the diversity of morphologic and functional types.

AB - 1. 1. Nematocyst structural proteins (NSP) from the sea anemones Aiptasia pallida and Metridium senile and the siphonophore Physalia physalis are primarily low molecular weight collagens linked by disulfide bonds. 2. 2. NSP patterns resolved by SDS-PAGE revealed a common, major collagen species (40 kDa) in each nematocyst type, together with other collagens and non-thiol-containing proteins. 3. 3. For each cnidarian, NSP glycosylation profiles were significantly different. 4. 4. Monoclonal antibodies against Aiptasia NSP demonstrated a differential distribution between capsule wall and thread. 5. 5. NSP differences would account for the diversity of morphologic and functional types.

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