Collagenase expression by normal human eosinophils

Boris V. Shlopov, Karen Hasty

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Collagenolytic activity was detected in extracts from human blood eosinophilic granulocytes. To characterize this collagenase, we compared extracts from isolated populations of eosinophils and neutrophils. Significant collagenase activity against type I and II collagens was present in extracts from both cell populations. Although collagenase activity was present in eosinophils, the cells did not stain with antibodies specific for fibroblast, neutrophil collagenase, or collagenase-3. In contrast, neutrophils immunostained positively with antibody to neutrophil collagenase. Western blot analysis confirmed the presence of immunoreactive protein in neutrophil extracts but not in the eosinophil extracts. Reverse transcription-polymerase chain reaction using primers specific for all three known collagenases of an eosinophil cell suspension from peripheral blood that had 3% contamination with immature neutrophils showed a polymerase chain reaction product only with neutrophil collagenase oligonucleotide primers, but not with fibroblast collagenase or collagenase-3 primers. Eosinophil collagenase would appear to have a unique antigenic structure and may represent a new enzyme.

Original languageEnglish (US)
Pages (from-to)451-455
Number of pages5
JournalJournal of Leukocyte Biology
Volume63
Issue number4
DOIs
StatePublished - Jan 1 1998

Fingerprint

Matrix Metalloproteinase 8
Collagenases
Eosinophils
Neutrophils
Matrix Metalloproteinase 13
Polymerase Chain Reaction
DNA Primers
Collagen Type II
Antibodies
Collagen Type I
Cell Extracts
Granulocytes
Population
Reverse Transcription
Suspensions
Coloring Agents
Western Blotting
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Cell Biology

Cite this

Collagenase expression by normal human eosinophils. / Shlopov, Boris V.; Hasty, Karen.

In: Journal of Leukocyte Biology, Vol. 63, No. 4, 01.01.1998, p. 451-455.

Research output: Contribution to journalArticle

Shlopov, Boris V. ; Hasty, Karen. / Collagenase expression by normal human eosinophils. In: Journal of Leukocyte Biology. 1998 ; Vol. 63, No. 4. pp. 451-455.
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