Comparative analysis of peptide p5 and serum amyloid P component for imaging AA amyloid in mice using dual-isotope SPECT

Jonathan Wall, Tina Richey, Angela Williams, Alan Stuckey, Dustin Osborne, Emily Martin, Stephen Kennel

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Purpose: I-labeled human serum amyloid P component (SAP) is used clinically only in the UK for imaging visceral amyloidosis to assist with diagnosis, disease staging, and monitoring response to therapy. We compare a new amyloid-reactive probe, peptide p5, with SAP for imaging amyloidosis. Procedures: Dual-energy SPECT/CT images were acquired of 125I-labeled SAP and 99mTclabeled p5 in mice with systemic AA amyloidosis (n=3). Twelve organs and tissues were harvested for radiotracer biodistribution assessment and for micro-autoradiographic analysis. Results: I-SAP and 99mTc-p5 localized equivalently in amyloid deposits in liver (-10% injected dose (ID)/g) whereas 125I-SAP was twofold higher in the spleen (-20% ID/g; 99mTc-p5, -10% ID/g). In contrast, 99mTc-p5 was bound to pancreatic and intestinal amyloid approximately fivefold more efficiently as evidenced in biodistribution data. Conclusions: Radiolabeled p5 is an effective amyloid-imaging radiotracer as compared to SAP in the murine model of amyloidosis and may be rapidly translated for imaging patients with visceral amyloidosis in the USA.

Original languageEnglish (US)
Pages (from-to)402-407
Number of pages6
JournalMolecular Imaging and Biology
Volume14
Issue number4
DOIs
StatePublished - Aug 1 2012

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Serum Amyloid P-Component
Single-Photon Emission-Computed Tomography
Amyloid
Isotopes
Amyloidosis
Peptides
Amyloid Plaques
Spleen
Liver

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging

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Comparative analysis of peptide p5 and serum amyloid P component for imaging AA amyloid in mice using dual-isotope SPECT. / Wall, Jonathan; Richey, Tina; Williams, Angela; Stuckey, Alan; Osborne, Dustin; Martin, Emily; Kennel, Stephen.

In: Molecular Imaging and Biology, Vol. 14, No. 4, 01.08.2012, p. 402-407.

Research output: Contribution to journalArticle

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AU - Richey, Tina

AU - Williams, Angela

AU - Stuckey, Alan

AU - Osborne, Dustin

AU - Martin, Emily

AU - Kennel, Stephen

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