Comparative mutagenic effects of structurally similar flavonoids quercetin and taxifolin on tester strains Salmonella typhimurium TA102 and Escherichia coli WP-2 uvrA

Patrudu S. Makena, Samuel C. Pierce, King Thom Chung, Scott Sinclair

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Quercetin (QT) and Taxifolin (TF) are structurally similar plant polyphenols. Both have been reported to have therapeutic potential as anti-cancer drugs and antioxidants. Mutagenic effects of QT and TF were evaluated using Salmonella typhimurium TA102 and Escherichia coli WP-2 uvrA tester strains. Either in the presence or absence of S9 mix, QT was mutagenic to TA102 and WP2 uvrA. However, the mutagenicity of QT was significantly enhanced in the presence of S9 mix. Likewise, in the presence of Iron (Fe2+) and NADPH generating system (NGS) and absence of S9 mix, QT induced significantly high mutations in both TA102 and WP-2 uvrA. Mutagenicity of QT decreased in both strains in the presence of Iron (Fe21) or NGS alone. TF was not mutagenic in the presence or absence of S9 mix in both TA102 and WP-2 uvrA 2, regardless of the presence of iron or NGS. Incorporation of antioxidants (ascorbate, superoxide dismutase (SOD), catalase (CAT)) and/or iron chelators (desferroxamine (DF) and ethylenediamine-tetraacetate (EDTA)) in the test systems markedly decreased QT-induced mutations in both tester strains. These results suggest that QT but not TF, could induce mutations in the presence or absence of rat liver S9 or Iron (Fe2+) and NGS in both tester strains by redox cycling and Fenton reactions to produce oxygen free radicals. Our results indicate that a minor structural variation between the two plant polyphenols could elicit a marked difference in their genotoxicities. These results provide a basis for further study into the potential use of QT in combination with iron supplements.

Original languageEnglish (US)
Pages (from-to)451-459
Number of pages9
JournalEnvironmental and Molecular Mutagenesis
Volume50
Issue number6
DOIs
StatePublished - Sep 22 2009

Fingerprint

Quercetin
Salmonella typhimurium
Flavonoids
Escherichia coli
NADP
Iron
ethylenediamine
Polyphenols
Mutation
Antioxidants
taxifolin
Deferoxamine
Chelating Agents
Catalase
Superoxide Dismutase
Oxidation-Reduction
Free Radicals
Reactive Oxygen Species
Liver

All Science Journal Classification (ASJC) codes

  • Health, Toxicology and Mutagenesis
  • Epidemiology
  • Genetics(clinical)

Cite this

Comparative mutagenic effects of structurally similar flavonoids quercetin and taxifolin on tester strains Salmonella typhimurium TA102 and Escherichia coli WP-2 uvrA. / Makena, Patrudu S.; Pierce, Samuel C.; Chung, King Thom; Sinclair, Scott.

In: Environmental and Molecular Mutagenesis, Vol. 50, No. 6, 22.09.2009, p. 451-459.

Research output: Contribution to journalArticle

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