Comparing different enamel pretreatment options for resin-infiltration of natural non-cavitated carious lesions

Marwa Abdelaziz, Adele Lodi Rizzini, Tissiana Bortolotto, Giovanni Tomasso Rocca, Albert J. Feilzer, Franklin Garcia-Godoy, Ivo Krejci

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Purpose: To compare two different enamel pretreatments and their effect on the efficiency of penetration of a one-component adhesive into natural carious lesions. Methods: Eight extracted human molars and premolars with non-cavitated interproximal lesions were selected. ICDAS code 1-2 was assessed by visual, microscopic, X-ray and Diagnocam record analysis. Samples were cut vertically across the demineralization to obtain two symmetrical lesions, (n=16). After isolating the cut surfaces with nail varnish, paired lesion halves' surfaces were pretreated with two different techniques: Group 1: surfaces were firstly abraded with fine diamond-coated metallic strips (Steelcarbo) and then etched with 37% H3PO4 acid (Omni-etch, 120 seconds); Group 2: lesion surfaces were etched with 15% HCl acid (Icon-etch, 120 seconds). All teeth were stained with rhodamine isothiocyanate (RITC) solution (12 hours) and subsequently stored in dry chamber (3 hours). All samples were penetrated with a one-component adhesive (Scotchbond Universal) for 180 seconds and coated with a thin layer of flowable composite (Tetric Flow). After light curing, unencapsulated dye was bleached by immersion in 30% hydogen peroxide for 12 hours at 37°C. Remaining lesion pores were stained with sodium fluorescein solution. Thin cuts of the teeth were observed with confocal microscopy and computer image analysis was performed (ImageJ). The percentage of penetration (area of resin penetration/area of total demineralization x 100) was calculated. Results: Pretreatment with fine aluminum oxide-coated metallic strip followed by 37% H3PO4 acid showed a larger infiltration area (51.7% ± 12.2) in almost all samples compared to pretreatment with 15% HCl acid alone (22.1% ± 13.2). Statistical analysis using t-test showed a significant difference between the two groups (P= 0.011).

Original languageEnglish (US)
Pages (from-to)3-9
Number of pages7
JournalAmerican Journal of Dentistry
Volume29
Issue number1
StatePublished - Feb 1 2016

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Dental Enamel
Acids
Tooth
Diamond
Paint
Aluminum Oxide
Bicuspid
Peroxides
Immersion
Nails
Fluorescein
Confocal Microscopy
Adhesives
Coloring Agents
X-Rays
Light

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Abdelaziz, M., Rizzini, A. L., Bortolotto, T., Rocca, G. T., Feilzer, A. J., Garcia-Godoy, F., & Krejci, I. (2016). Comparing different enamel pretreatment options for resin-infiltration of natural non-cavitated carious lesions. American Journal of Dentistry, 29(1), 3-9.

Comparing different enamel pretreatment options for resin-infiltration of natural non-cavitated carious lesions. / Abdelaziz, Marwa; Rizzini, Adele Lodi; Bortolotto, Tissiana; Rocca, Giovanni Tomasso; Feilzer, Albert J.; Garcia-Godoy, Franklin; Krejci, Ivo.

In: American Journal of Dentistry, Vol. 29, No. 1, 01.02.2016, p. 3-9.

Research output: Contribution to journalArticle

Abdelaziz, M, Rizzini, AL, Bortolotto, T, Rocca, GT, Feilzer, AJ, Garcia-Godoy, F & Krejci, I 2016, 'Comparing different enamel pretreatment options for resin-infiltration of natural non-cavitated carious lesions', American Journal of Dentistry, vol. 29, no. 1, pp. 3-9.
Abdelaziz, Marwa ; Rizzini, Adele Lodi ; Bortolotto, Tissiana ; Rocca, Giovanni Tomasso ; Feilzer, Albert J. ; Garcia-Godoy, Franklin ; Krejci, Ivo. / Comparing different enamel pretreatment options for resin-infiltration of natural non-cavitated carious lesions. In: American Journal of Dentistry. 2016 ; Vol. 29, No. 1. pp. 3-9.
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abstract = "Purpose: To compare two different enamel pretreatments and their effect on the efficiency of penetration of a one-component adhesive into natural carious lesions. Methods: Eight extracted human molars and premolars with non-cavitated interproximal lesions were selected. ICDAS code 1-2 was assessed by visual, microscopic, X-ray and Diagnocam record analysis. Samples were cut vertically across the demineralization to obtain two symmetrical lesions, (n=16). After isolating the cut surfaces with nail varnish, paired lesion halves' surfaces were pretreated with two different techniques: Group 1: surfaces were firstly abraded with fine diamond-coated metallic strips (Steelcarbo) and then etched with 37{\%} H3PO4 acid (Omni-etch, 120 seconds); Group 2: lesion surfaces were etched with 15{\%} HCl acid (Icon-etch, 120 seconds). All teeth were stained with rhodamine isothiocyanate (RITC) solution (12 hours) and subsequently stored in dry chamber (3 hours). All samples were penetrated with a one-component adhesive (Scotchbond Universal) for 180 seconds and coated with a thin layer of flowable composite (Tetric Flow). After light curing, unencapsulated dye was bleached by immersion in 30{\%} hydogen peroxide for 12 hours at 37°C. Remaining lesion pores were stained with sodium fluorescein solution. Thin cuts of the teeth were observed with confocal microscopy and computer image analysis was performed (ImageJ). The percentage of penetration (area of resin penetration/area of total demineralization x 100) was calculated. Results: Pretreatment with fine aluminum oxide-coated metallic strip followed by 37{\%} H3PO4 acid showed a larger infiltration area (51.7{\%} ± 12.2) in almost all samples compared to pretreatment with 15{\%} HCl acid alone (22.1{\%} ± 13.2). Statistical analysis using t-test showed a significant difference between the two groups (P= 0.011).",
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