Comparing flow cytometric analysis and nucleolar organizer region enumeration in archival oral premalignant lesions

M. A. Kahn, H. H. Mincer, M. E. Dockter, Jeanne Hermann-Petrin

    Research output: Contribution to journalArticle

    24 Citations (Scopus)

    Abstract

    Flow cytometric analysis (FCA) and silver colloidal nucleolar organizer region‐associated protein staining (AgNOR) have been used individually in assessing the histopathologic nature of various human tumors. However, few researchers have investigated the relationship between the two techniques in a single series. In a retrospective study, we examined 36 premalignant lesions of the oral cavity by FCA and AgNOR on formalin‐fixed, paraffin‐embedded tissue submitted to the University of Tennessee, Memphis, oral pathology laboratory. Three categories of epithelial dysplasia were represented (9 mild. 9 moderate, 6 severe), as well as four epithelial hyperplasias without dysplasia, three squamous cell carcinomas, and five fibrous nodules as controls. Parameters recorded for each case included age. race, gender, site, light microscopic diagnosis (LMD). DNA index (DI), total proliferative index (TPI), S‐phase (S), range of nucleolar organizer regions (RNOR), and mean number of nucleolar organizer regions (MNOR). The average maximum nucleolar organizer region count (AMXNOR) for each LMD category was also calculated. The objective of the study was to determine if FCA or AgNOR aided in the subjective LMD of oral premalignant lesions and if the parameters recorded for the specimens exhibited any positive correlation. The FCA results indicated an abnormal DI in 6 of the 24 dysplastic lesions. A positive partial correlation was seen between DI and MNOR (r = 0.434: P<0.012) and TPI and S (r = 0.774; P < 0.0001), holding gender and race constant. Additionally, the AMXNOR exhibited a slight tendency to increase for each increasing grade of dysplasia but this could not be confirmed statistically. The results indicate that there is a positive correlation between FCA and AgNOR but that neither FCA nor AgNOR were a diagnostic aid in grading oral mucosal dysplasia.

    Original languageEnglish (US)
    Pages (from-to)257-262
    Number of pages6
    JournalJournal of Oral Pathology & Medicine
    Volume22
    Issue number6
    DOIs
    StatePublished - Jan 1 1993

    Fingerprint

    Nucleolus Organizer Region
    Light
    DNA
    Oral Pathology
    Oral Diagnosis
    Hyperplasia
    Mouth
    Squamous Cell Carcinoma
    Retrospective Studies
    Research Personnel
    nucleolar organizer region associated proteins
    Staining and Labeling
    Neoplasms
    Proteins

    All Science Journal Classification (ASJC) codes

    • Pathology and Forensic Medicine
    • Oral Surgery
    • Otorhinolaryngology
    • Cancer Research
    • Periodontics

    Cite this

    Comparing flow cytometric analysis and nucleolar organizer region enumeration in archival oral premalignant lesions. / Kahn, M. A.; Mincer, H. H.; Dockter, M. E.; Hermann-Petrin, Jeanne.

    In: Journal of Oral Pathology & Medicine, Vol. 22, No. 6, 01.01.1993, p. 257-262.

    Research output: Contribution to journalArticle

    Kahn, M. A. ; Mincer, H. H. ; Dockter, M. E. ; Hermann-Petrin, Jeanne. / Comparing flow cytometric analysis and nucleolar organizer region enumeration in archival oral premalignant lesions. In: Journal of Oral Pathology & Medicine. 1993 ; Vol. 22, No. 6. pp. 257-262.
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    abstract = "Flow cytometric analysis (FCA) and silver colloidal nucleolar organizer region‐associated protein staining (AgNOR) have been used individually in assessing the histopathologic nature of various human tumors. However, few researchers have investigated the relationship between the two techniques in a single series. In a retrospective study, we examined 36 premalignant lesions of the oral cavity by FCA and AgNOR on formalin‐fixed, paraffin‐embedded tissue submitted to the University of Tennessee, Memphis, oral pathology laboratory. Three categories of epithelial dysplasia were represented (9 mild. 9 moderate, 6 severe), as well as four epithelial hyperplasias without dysplasia, three squamous cell carcinomas, and five fibrous nodules as controls. Parameters recorded for each case included age. race, gender, site, light microscopic diagnosis (LMD). DNA index (DI), total proliferative index (TPI), S‐phase (S), range of nucleolar organizer regions (RNOR), and mean number of nucleolar organizer regions (MNOR). The average maximum nucleolar organizer region count (AMXNOR) for each LMD category was also calculated. The objective of the study was to determine if FCA or AgNOR aided in the subjective LMD of oral premalignant lesions and if the parameters recorded for the specimens exhibited any positive correlation. The FCA results indicated an abnormal DI in 6 of the 24 dysplastic lesions. A positive partial correlation was seen between DI and MNOR (r = 0.434: P<0.012) and TPI and S (r = 0.774; P < 0.0001), holding gender and race constant. Additionally, the AMXNOR exhibited a slight tendency to increase for each increasing grade of dysplasia but this could not be confirmed statistically. The results indicate that there is a positive correlation between FCA and AgNOR but that neither FCA nor AgNOR were a diagnostic aid in grading oral mucosal dysplasia.",
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