Comparison of immunohistochemistry and silver stain for the diagnosis of pediatric Helicobacter pylori infection in urease-negative gastric biopsies

John K. Eshun, Dennis Black, Helen B. Casteel, Hazel Horn, Toni Beavers-May, Christina A. Jetton, David M. Parham

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Abstract

We compared immunohistochemical and silver stains of pediatric gastric biopsy sections for the identification of Helicobacter pylori infection with chronic inflammation and a negative urease screening test. Thirty-seven patients (age range 10 months to 21 years) whose gastric antral biopsies were negative for the rapid urease test (CLOR) but positive for lymphocytic infiltration were selected for a retrospective study. Specimens had been subjected to a rapid urease test (CLOR) and hematoxylin and eosin staining, and Dieterle silver staining and immunohistochemical staining specific for H. pylori were also performed. Twelve additional patients with ureasepositive biopsies were used as controls. With Dieterle staining, 8/37 (22%) urease-negative biopsies contained organisms morphologically compatible with H. pylori, 21/37 (56%) contained organisms not compatible with H. pylori, and 8/37 (22%) were negative for organisms. Immunostaining confirmed 6/8 (75%) Dieterle-positive cases as being H. pylori, was negative in 2/8 (25%) Dieterle-positive cases, and was positive in 2/8 (25%) Dieterle-negative cases. Biopsies from 8/12 (67%) ureasepositive specimens contained organisms seen with both Dieterle and immunohistochemical stains, and 4/12 (33%) were negative with both stains. Although both stains yielded comparable results with H. pylori-positive biopsies, Dieterle staining was potentially confusing because of nonspecific staining of other organisms. A significant proportion of (CLOR)-negative biopsies was positive for H. pylori with special stains. We therefore recommend the use of immunohistochemical staining rather than silver staining in the evaluation of ureasenegative gastric biopsies demonstrating chronic inflammation in children.

Original languageEnglish (US)
Pages (from-to)82-88
Number of pages7
JournalPediatric and Developmental Pathology
Volume4
Issue number1
DOIs
StatePublished - Feb 26 2001

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Urease
Helicobacter Infections
Silver
Helicobacter pylori
Stomach
Coloring Agents
Immunohistochemistry
Pediatrics
Biopsy
Staining and Labeling
Silver Staining
Inflammation
Hematoxylin
Eosine Yellowish-(YS)
Retrospective Studies

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health
  • Pathology and Forensic Medicine

Cite this

Comparison of immunohistochemistry and silver stain for the diagnosis of pediatric Helicobacter pylori infection in urease-negative gastric biopsies. / Eshun, John K.; Black, Dennis; Casteel, Helen B.; Horn, Hazel; Beavers-May, Toni; Jetton, Christina A.; Parham, David M.

In: Pediatric and Developmental Pathology, Vol. 4, No. 1, 26.02.2001, p. 82-88.

Research output: Contribution to journalArticle

Eshun, John K. ; Black, Dennis ; Casteel, Helen B. ; Horn, Hazel ; Beavers-May, Toni ; Jetton, Christina A. ; Parham, David M. / Comparison of immunohistochemistry and silver stain for the diagnosis of pediatric Helicobacter pylori infection in urease-negative gastric biopsies. In: Pediatric and Developmental Pathology. 2001 ; Vol. 4, No. 1. pp. 82-88.
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abstract = "We compared immunohistochemical and silver stains of pediatric gastric biopsy sections for the identification of Helicobacter pylori infection with chronic inflammation and a negative urease screening test. Thirty-seven patients (age range 10 months to 21 years) whose gastric antral biopsies were negative for the rapid urease test (CLOR) but positive for lymphocytic infiltration were selected for a retrospective study. Specimens had been subjected to a rapid urease test (CLOR) and hematoxylin and eosin staining, and Dieterle silver staining and immunohistochemical staining specific for H. pylori were also performed. Twelve additional patients with ureasepositive biopsies were used as controls. With Dieterle staining, 8/37 (22{\%}) urease-negative biopsies contained organisms morphologically compatible with H. pylori, 21/37 (56{\%}) contained organisms not compatible with H. pylori, and 8/37 (22{\%}) were negative for organisms. Immunostaining confirmed 6/8 (75{\%}) Dieterle-positive cases as being H. pylori, was negative in 2/8 (25{\%}) Dieterle-positive cases, and was positive in 2/8 (25{\%}) Dieterle-negative cases. Biopsies from 8/12 (67{\%}) ureasepositive specimens contained organisms seen with both Dieterle and immunohistochemical stains, and 4/12 (33{\%}) were negative with both stains. Although both stains yielded comparable results with H. pylori-positive biopsies, Dieterle staining was potentially confusing because of nonspecific staining of other organisms. A significant proportion of (CLOR)-negative biopsies was positive for H. pylori with special stains. We therefore recommend the use of immunohistochemical staining rather than silver staining in the evaluation of ureasenegative gastric biopsies demonstrating chronic inflammation in children.",
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