Complement factor H

Spatial and temporal expression and localization in the eye

Nawajes Mandal, Radha Ayyagari

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

PURPOSE. Complement factor H (CFH) is a component of the mammalian complement system, which regulates the alternative pathway of complement activation and protects the host cell from inappropriate complement activation. CFH is a key regulator of innate immunity, and CFH deficiency leads to membranoproliferative glomerulonephritis type II. A variation in human CFH, Y402H, has been shown to be associated with an increased risk for age-related macular degeneration. The authors describe studies on the spatial and temporal expression of the CFH gene and localization of this protein in ocular tissues to gain insight into its role in the eye. METHODS. CFH expression in human and mouse tissues was studied by quantitative RT-PCR and Western blot analysis, and localization of CFH was studied by immunohistochemical analysis followed by fluorescence microscopy. RESULTS. In human and mouse, CFH expression was found to be similar to the highest level of expression in the liver. In ocular tissue, CFH was detected in the distalmost optic nerve (3 mm) cut from the scleral surface of the eyeball, sclera, RPE-choroid, retina, lens, and ciliary body. In mouse, Cfh expression was observed from early embryonic stages, and in the eye its expression increased with age. CONCLUSIONS. A significant level of CFH expression is maintained in different ocular tissues during development and aging. Sustained high levels of CFH expression in eye tissues suggest that this protein may play a role in protecting these tissues from indiscriminate complement activation and inflammatory insult.

Original languageEnglish (US)
Pages (from-to)4091-4097
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume47
Issue number9
DOIs
StatePublished - Sep 1 2006
Externally publishedYes

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Complement Factor H
Complement Activation
Membranoproliferative Glomerulonephritis
Alternative Complement Pathway
Ciliary Body
Sclera
Choroid
Macular Degeneration
Optic Nerve
Fluorescence Microscopy
Innate Immunity
Lenses
Retina
Proteins
Western Blotting
Polymerase Chain Reaction
Liver

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

Complement factor H : Spatial and temporal expression and localization in the eye. / Mandal, Nawajes; Ayyagari, Radha.

In: Investigative Ophthalmology and Visual Science, Vol. 47, No. 9, 01.09.2006, p. 4091-4097.

Research output: Contribution to journalArticle

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