Complete replication of hepatitis B virus and hepatitis C virus in a newly developed hepatoma cell line

Darong Yang, Chaohui Zuo, Xiaohong Wang, Xianghe Meng, Binbin Xue, Nianli Liu, Rong Yu, Yuwen Qin, Yimin Gao, Qiuping Wang, Jun Hu, Ling Wang, Zebin Zhou, Bing Liu, Deming Tan, Yang Guan, Haizhen Zhu

Research output: Contribution to journalArticle

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Abstract

The absence of a robust cell culture system for hepatitis B virus (HBV) and hepatitis C virus (HCV) infection has limited the analysis of the virus lifecycle and drug discovery. We have established a hepatoma cell line, HLCZ01, the first cell line, to the authors' knowledge, supporting the entire lifecycle of both HBV and HCV. HBV surface antigen (HBsAg)-positive particles can be observed in the supernatant and the lumen of the endoplasmic reticulum of the cells via electron microscopy. Interestingly, HBV and HCV clinical isolates propagate in HLCZ01 cells. Both viruses replicate in the cells without evidence of overt interference. HBV and HCV entry are blocked by antibodies against HBsAg and human CD81, respectively, and the replication of HBV and HCV is inhibited by antivirals. HLCZ01 cells mount an innate immune response to virus infection. The cell line provides a powerful tool for exploring the mechanisms of virus entry and replication and the interaction between host and virus, facilitating the development of novel antiviral agents and vaccines.

Original languageEnglish (US)
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number13
DOIs
StatePublished - Apr 1 2014

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Hepatitis B virus
Hepacivirus
Hepatocellular Carcinoma
Cell Line
Virus Internalization
Virus Diseases
Viruses
Antiviral Agents
Drug Discovery
Surface Antigens
Hepatitis B Surface Antigens
Virus Replication
Innate Immunity
Endoplasmic Reticulum
Electron Microscopy
Vaccines
Cell Culture Techniques
Antibodies

All Science Journal Classification (ASJC) codes

  • General

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Complete replication of hepatitis B virus and hepatitis C virus in a newly developed hepatoma cell line. / Yang, Darong; Zuo, Chaohui; Wang, Xiaohong; Meng, Xianghe; Xue, Binbin; Liu, Nianli; Yu, Rong; Qin, Yuwen; Gao, Yimin; Wang, Qiuping; Hu, Jun; Wang, Ling; Zhou, Zebin; Liu, Bing; Tan, Deming; Guan, Yang; Zhu, Haizhen.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 13, 01.04.2014.

Research output: Contribution to journalArticle

Yang, D, Zuo, C, Wang, X, Meng, X, Xue, B, Liu, N, Yu, R, Qin, Y, Gao, Y, Wang, Q, Hu, J, Wang, L, Zhou, Z, Liu, B, Tan, D, Guan, Y & Zhu, H 2014, 'Complete replication of hepatitis B virus and hepatitis C virus in a newly developed hepatoma cell line', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 13. https://doi.org/10.1073/pnas.1320071111
Yang, Darong ; Zuo, Chaohui ; Wang, Xiaohong ; Meng, Xianghe ; Xue, Binbin ; Liu, Nianli ; Yu, Rong ; Qin, Yuwen ; Gao, Yimin ; Wang, Qiuping ; Hu, Jun ; Wang, Ling ; Zhou, Zebin ; Liu, Bing ; Tan, Deming ; Guan, Yang ; Zhu, Haizhen. / Complete replication of hepatitis B virus and hepatitis C virus in a newly developed hepatoma cell line. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 13.
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