Complete Sequence of pCY-CTX, a Plasmid Carrying a Phage-Like Region and an ISEcp1-Mediated Tn2 Element from Enterobacter cloacae

Zhenbo Xu, Jinhong Xie, Ling Yang, Dingqiang Chen, Brian Peters, Mark E. Shirtliff

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A plasmid pCY-CTX carrying a phage-like backbone from an extensively drug-resistant Enterobacter cloacae strain Guangzhou-ECL001 (previously known as CY01) was identified in this study. By Illumina MiSeq 2 × 250-bp paired-end sequencing, de novo assembly, and PCR, full sequence of pCY-CTX was obtained. Plasmid pCY-CTX was a circular plasmid with a length of 116,700 bp, harboring 136 putative open reading frames with the average G + C content of 50.8%. The backbone of pCY-CTX showed high identity to previously reported phage-like plasmid pHCM2 and phage SSU5. In addition, pCY-CTX contained a distinctive ISEcp1-mediated Tn2 region with two resistance genes blaTEM-1 and blaCTX-M-3. Transposition unit "ISEcp1- blaCTX-M-3- orf477" was inserted into the Tn2 structure, dividing Tn2 into two parts. This represents the first identification of a plasmid carrying a phage-like backbone and a distinctive ISEcp1-mediated Tn2 region within blaTEM-1 and blaCTX-M-3 in clinical E. cloacae. The finding of phage-like regions located in plasmids provides a new perspective in gene transfer associated with antimicrobial resistance.

Original languageEnglish (US)
Pages (from-to)307-313
Number of pages7
JournalMicrobial Drug Resistance
Volume24
Issue number3
DOIs
StatePublished - Apr 1 2018

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Enterobacter cloacae
Bacteriophages
Plasmids
Base Composition
Open Reading Frames
Genes
Polymerase Chain Reaction
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Pharmacology
  • Microbiology (medical)

Cite this

Complete Sequence of pCY-CTX, a Plasmid Carrying a Phage-Like Region and an ISEcp1-Mediated Tn2 Element from Enterobacter cloacae. / Xu, Zhenbo; Xie, Jinhong; Yang, Ling; Chen, Dingqiang; Peters, Brian; Shirtliff, Mark E.

In: Microbial Drug Resistance, Vol. 24, No. 3, 01.04.2018, p. 307-313.

Research output: Contribution to journalArticle

Xu, Zhenbo ; Xie, Jinhong ; Yang, Ling ; Chen, Dingqiang ; Peters, Brian ; Shirtliff, Mark E. / Complete Sequence of pCY-CTX, a Plasmid Carrying a Phage-Like Region and an ISEcp1-Mediated Tn2 Element from Enterobacter cloacae. In: Microbial Drug Resistance. 2018 ; Vol. 24, No. 3. pp. 307-313.
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