Components of Golgi-to-vacuole trafficking are required for nitrogen- and TORC1-responsive regulation of the yeast GATA factors

Mohammad Fayyadkazan, Jennifer J. Tate, Fabienne Vierendeels, Terrance Cooper, Evelyne Dubois, Isabelle Georis

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Nitrogen catabolite repression (NCR) is the regulatory pathway through which Saccharomyces cerevisiae responds to the available nitrogen status and selectively utilizes rich nitrogen sources in preference to poor ones. Expression of NCR-sensitive genes is mediated by two transcription activators, Gln3 and Gat1, in response to provision of a poorly used nitrogen source or following treatment with the TORC1 inhibitor, rapamycin. During nitrogen excess, the transcription activators are sequestered in the cytoplasm in a Ure2-dependent fashion. Here, we show that Vps components are required for Gln3 localization and function in response to rapamycin treatment when cells are grown in defined yeast nitrogen base but not in complex yeast peptone dextrose medium. On the other hand, Gat1 function was altered in vps mutants in all conditions tested. A significant fraction of Gat1, like Gln3, is associated with light intracellular membranes. Further, our results are consistent with the possibility that Ure2 might function downstream of the Vps components during the control of GATA factor-mediated gene expression. These observations demonstrate distinct media-dependent requirements of vesicular trafficking components for wild-type responses of GATA factor localization and function. As a result, the current model describing participation of Vps system components in events associated with translocation of Gln3 into the nucleus following rapamycin treatment or growth in nitrogen-poor medium requires modification.

Original languageEnglish (US)
Pages (from-to)271-287
Number of pages17
JournalMicrobiologyOpen
Volume3
Issue number3
DOIs
StatePublished - Jan 1 2014

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GATA Transcription Factors
Vacuoles
Nitrogen
Yeasts
Sirolimus
Catabolite Repression
Peptones
Intracellular Membranes
mechanistic target of rapamycin complex 1
Saccharomyces cerevisiae
Cytoplasm
Gene Expression
Glucose

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Components of Golgi-to-vacuole trafficking are required for nitrogen- and TORC1-responsive regulation of the yeast GATA factors. / Fayyadkazan, Mohammad; Tate, Jennifer J.; Vierendeels, Fabienne; Cooper, Terrance; Dubois, Evelyne; Georis, Isabelle.

In: MicrobiologyOpen, Vol. 3, No. 3, 01.01.2014, p. 271-287.

Research output: Contribution to journalArticle

Fayyadkazan, Mohammad ; Tate, Jennifer J. ; Vierendeels, Fabienne ; Cooper, Terrance ; Dubois, Evelyne ; Georis, Isabelle. / Components of Golgi-to-vacuole trafficking are required for nitrogen- and TORC1-responsive regulation of the yeast GATA factors. In: MicrobiologyOpen. 2014 ; Vol. 3, No. 3. pp. 271-287.
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