Compound 49b protects against blast-induced retinal injury

Youde Jiang, Li Liu, Jayaprakash Pagadala, Duane Miller, Jena J. Steinle

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aim: To determine whether Compound 49b, a novel beta-adrenergic receptor agonist, can prevent increased inflammation and apoptosis in mice after exposure to ocular blast.Methods: Eyes of C57/BL6 mice were exposed to a blast of air from a paintball gun at 26 psi (≈0.18 MPa). Eyes were collected 4 hours, 24 hours, and 72 hours after blast exposure. In a subset of mice, Compound 49b eyedrops (1 mM) were applied within 4 hours, 24 hours, or 72 hours of the blast. Three days after blast exposure, all mice were sacrificed. One eye was used to measure levels of retinal proteins (TNFα, IL-1β, Bax, BcL-xL, caspase 3, and cytochrome C). The other eye was used for TUNEL labeling of apoptotic cells, which were co-labeled with NeuN to stain for retinal ganglion cells.Results: We found that ocular exposure to 26 psi air pressure led to a significant increase in levels of apoptotic and inflammatory mediators within 4 hours, which lasted throughout the period investigated. When Compound 49b was applied within 4 hours or 24 hours of blast injury, levels of apoptotic and inflammatory mediators were significantly reduced. Application of Compound 49b within 72 hours of blast injury reduced levels of inflammatory mediators, but not to untreated levels.Conclusions: Ocular blast injury produces a significant increase in levels of key inflammatory and apoptotic markers in the retina as early as 4 hours after blast exposure. These levels are significantly reduced if a beta-adrenergic receptor agonist is applied within 24 hours of blast exposure. Data suggest that local application of beta-adrenergic receptor agonists may be beneficial to reduce inflammation and apoptosis.

Original languageEnglish (US)
Article number96
JournalJournal of Neuroinflammation
Volume10
DOIs
StatePublished - Jul 30 2013

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Blast Injuries
Adrenergic beta-Agonists
Wounds and Injuries
Apoptosis
Inflammation
Air Pressure
Eye Injuries
Retinal Ganglion Cells
Ophthalmic Solutions
In Situ Nick-End Labeling
Firearms
Cytochromes
Interleukin-1
Caspase 3
Retina
Coloring Agents
Air
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology
  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Compound 49b protects against blast-induced retinal injury. / Jiang, Youde; Liu, Li; Pagadala, Jayaprakash; Miller, Duane; Steinle, Jena J.

In: Journal of Neuroinflammation, Vol. 10, 96, 30.07.2013.

Research output: Contribution to journalArticle

Jiang, Youde ; Liu, Li ; Pagadala, Jayaprakash ; Miller, Duane ; Steinle, Jena J. / Compound 49b protects against blast-induced retinal injury. In: Journal of Neuroinflammation. 2013 ; Vol. 10.
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