Comprehensive seroprofiling of sixteen B. burgdorferi OspC

Implications for Lyme disease diagnostics design

Larisa Ivanova, Iva Christova, Vera Neves, Miguel Aroso, Luciana Meirelles, Dustin Brisson, Maria Gomes-Solecki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Early diagnosis of Lyme disease (LD) is critical to successful treatment. However, current serodiagnostic tests do not reliably detect antibodies during early infection. OspC induces a potent early immune response and is also one of the most diverse proteins in the Borrelia proteome. Yet, at least 70% of the amino acid sequence is conserved among all 21 known OspC types. We performed a series of comprehensive seroprofiling studies to select the OspC types that have the most cross-reactive immunodominant epitopes. We found that proteins belonging to seven OspC types detect antibodies from all three infected host species regardless of the OspC genotype of the infecting strain. Although no one OspC type identifies all seropositive human samples, combinations of as few as two OspC proteins identified all patients that had anti-OspC antibodies.

Original languageEnglish (US)
Pages (from-to)393-400
Number of pages8
JournalClinical Immunology
Volume132
Issue number3
DOIs
StatePublished - Sep 1 2009

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Lyme Disease
Borrelia
Immunodominant Epitopes
Antibodies
Proteome
Early Diagnosis
Anti-Idiotypic Antibodies
Amino Acid Sequence
Proteins
Genotype
Infection
Therapeutics
OspC protein

All Science Journal Classification (ASJC) codes

  • Immunology
  • Immunology and Allergy

Cite this

Comprehensive seroprofiling of sixteen B. burgdorferi OspC : Implications for Lyme disease diagnostics design. / Ivanova, Larisa; Christova, Iva; Neves, Vera; Aroso, Miguel; Meirelles, Luciana; Brisson, Dustin; Gomes-Solecki, Maria.

In: Clinical Immunology, Vol. 132, No. 3, 01.09.2009, p. 393-400.

Research output: Contribution to journalArticle

Ivanova, Larisa ; Christova, Iva ; Neves, Vera ; Aroso, Miguel ; Meirelles, Luciana ; Brisson, Dustin ; Gomes-Solecki, Maria. / Comprehensive seroprofiling of sixteen B. burgdorferi OspC : Implications for Lyme disease diagnostics design. In: Clinical Immunology. 2009 ; Vol. 132, No. 3. pp. 393-400.
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