Congenital Lack of COX-2 Affects Mechanical and Geometric Properties of Bone in Mice

Q. Chen, J. Y. Rho, Z. Fan, S. J.F. Laulederkind, Rajendra Raghow

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We compared the mechanical properties of bones from mice lacking either a functional cycloxygenase-1 (C57BL6/DBA COX-1-/-; n = 9) or COX-2 (C57BL6/DBA COX-2-/-; n = 9) gene and wild type mice (C57BL6/DBA; n = 10). Twenty-eight right femora from 3-month-old male mice were used to determine bulk structural and material properties of bone by three-point bending. Bone matrix properties were also measured by nanoindentation to access the changes in bulk mechanical properties due to changes in bone matrix or bone geometry. The bulk material properties (elastic modulus, P < 0.05; ultimate stress, P < 0.01) of COX-2-/- bones were lower than those of wild-type mice whereas the bulk structural properties (stiffness, P > 0.2; breaking force, P > 0.1) were similar to those of the wild-type mice. COX-2-/- mice had a longer moment of inertia but their cortical bones were thinner and contained many more intra-cortical pores compared with the bones of the other two groups. Finally, the bone matrix properties of COX-1-/- mice, COX-2-/-mice and their heterozygous littermates were similar to those of C57BL6/DBA wild-type mice.

Original languageEnglish (US)
Pages (from-to)387-392
Number of pages6
JournalCalcified Tissue International
Volume73
Issue number4
DOIs
StatePublished - Oct 1 2003

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Bone and Bones
Bone Matrix
Inbred DBA Mouse
Elastic Modulus
Femur
Genes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Cite this

Congenital Lack of COX-2 Affects Mechanical and Geometric Properties of Bone in Mice. / Chen, Q.; Rho, J. Y.; Fan, Z.; Laulederkind, S. J.F.; Raghow, Rajendra.

In: Calcified Tissue International, Vol. 73, No. 4, 01.10.2003, p. 387-392.

Research output: Contribution to journalArticle

Chen, Q. ; Rho, J. Y. ; Fan, Z. ; Laulederkind, S. J.F. ; Raghow, Rajendra. / Congenital Lack of COX-2 Affects Mechanical and Geometric Properties of Bone in Mice. In: Calcified Tissue International. 2003 ; Vol. 73, No. 4. pp. 387-392.
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