Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice

Jianfeng Xiao, Mohammad Moshahid Khan, Satya Vemula, Jun Tian, Mark Ledoux

Research output: Contribution to journalArticle

Abstract

CIZ1 plays a role in DNA synthesis at the G1/S checkpoint. Ciz1 gene-trap null mice manifest motor dysfunction, cell-cycle abnormalities, and DNA damage. In contrast, it has previously been reported that mouse embryonic fibroblasts derived from presumed Ciz1 knock-out mice (Ciz1tm1.1Homy/tm1.1Homy) generated by crossing Cre-expressing mice with exon 5-floxed mice (Ciz1tm1Homy/tm1Homy) do not exhibit evidence of enhanced DNA damage following γ-irradiation or cell-cycle defects. Here, we report that Ciz1tm1.1Homy/tm1.1Homy mice show loss of Ciz1 exon 5 but are neurologically normal and express abnormal transcripts (Ciz1ΔE5/ΔE5 mice) that are translated into one or more proteins of approximate wild-type size. Therefore, Ciz1tm1.1Homy/tm1.1Homy mice (Ciz1ΔE5/ΔE5) lose residues encoded by exon 5 but may gain function from novel amino acid sequences.

Original languageEnglish (US)
Pages (from-to)3101-3110
Number of pages10
JournalFEBS Letters
Volume592
Issue number18
DOIs
StatePublished - Sep 1 2018

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Exons
DNA
Cells
Fibroblasts
DNA Damage
Genes
Irradiation
Cell Cycle
Amino Acids
Defects
Knockout Mice
Proteins
Amino Acid Sequence

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice. / Xiao, Jianfeng; Khan, Mohammad Moshahid; Vemula, Satya; Tian, Jun; Ledoux, Mark.

In: FEBS Letters, Vol. 592, No. 18, 01.09.2018, p. 3101-3110.

Research output: Contribution to journalArticle

Xiao, Jianfeng ; Khan, Mohammad Moshahid ; Vemula, Satya ; Tian, Jun ; Ledoux, Mark. / Consequences of Cre-mediated deletion of Ciz1 exon 5 in mice. In: FEBS Letters. 2018 ; Vol. 592, No. 18. pp. 3101-3110.
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