Conserved amino acids within CCAAT enhancer-binding proteins (C/EBPα and β) regulate phosphoenolpyruvate carboxykinase (PEPCK) gene expression

Luis A. Jurado, Shulan Song, William J. Roesler, Edwards Park

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Thyroid hormone and cAMP stimulate transcription of the gene for phosphoenolpyruvate carboxykinase (PEPCK). CCAAT enhancer-binding proteins (C/EBPα and β) are involved in multiple aspects of the nutritional, developmental and hormonal regulation of PEPCK gene expression. Previously, we have identified a thyroid hormone response element in the PEPCK promoter and demonstrated that C/EBP proteins bound to the P3(I) site are participants in the induction of PEPCK gene expression by thyroid hormone and cAMP. Here, we identify several peptide regions within the transactivation domain of C/EBPα that enhance the ability of T3 to stimulate gene transcription. We also demonstrate that several conserved amino acids in the transactivation domain of C/EBPα and C/EBP β are required for the stimulation of basal gene expression and identify amino acids within C/EBP β that participate in the cAMP induction of the PEPCK gene. Finally, we show that the CREB-binding protein (CBP) enhanced the induction of PEPCK gene transcription by thyroid hormone and that CBP is associated with the PEPCK gene in vivo. Our results indicate that both C/EBP proteins and CBP participate in the regulation of PEPCK gene transcription by thyroid hormone.

Original languageEnglish (US)
Pages (from-to)27606-27612
Number of pages7
JournalJournal of Biological Chemistry
Volume277
Issue number31
DOIs
StatePublished - Aug 2 2002

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CCAAT-Enhancer-Binding Proteins
Phosphoenolpyruvate
Protein C
Gene expression
Gene Expression
Amino Acids
Thyroid Hormones
Genes
CREB-Binding Protein
Transcription
Transcriptional Activation
Response Elements
Protein Binding
Peptides

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Conserved amino acids within CCAAT enhancer-binding proteins (C/EBPα and β) regulate phosphoenolpyruvate carboxykinase (PEPCK) gene expression. / Jurado, Luis A.; Song, Shulan; Roesler, William J.; Park, Edwards.

In: Journal of Biological Chemistry, Vol. 277, No. 31, 02.08.2002, p. 27606-27612.

Research output: Contribution to journalArticle

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