Constitutive and nitrogen catabolite repression-sensitive production of Gat1 isoforms

Rajendra Rai, Jennifer J. Tate, Isabelle Georis, Evelyne Dubois, Terrance Cooper

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Nitrogen catabolite repression (NCR)-sensitive transcription is activatedby Gln3 and Gat1. Innitrogen excess, Gln3 and Gat1 are cytoplasmic, and transcription is minimal. In poor nitrogen, Gln3 and Gat1 become nuclear and activate transcription. A long standing paradox has surrounded Gat1 production. Gat1 was first reported as an NCR-regulated activity mediating NCR-sensitive transcription in gln3 deletion strains. Upon cloning, GAT1 transcription was, as predicted, NCR-sensitive and Gln3- and Gat1-activated. In contrast, Western blots of Gat1-Myc13 exhibited two constitutively produced species. Investigating this paradox, wedemonstrate that wild type Gat1 isoforms (IsoA and IsoB) are initiated at Gat1 methionines 40, 95, and/or 102, but not at methionine 1. Their low level production is the same in rich and poor nitrogen conditions. When the Myc13 tag is placed after Gat1 Ser-233, four N-terminal Gat1 isoforms (IsoC-F) are also initiated at methionines 40, 95, and/or 102. However, their production is highly NCR-sensitive, being greater in proline than glutamine medium. Surprisingly, all Gat1 isoforms produced in sufficient quantities to be confidently analyzed (IsoA, IsoC, and IsoD) require Gln3 and UASGATA promoter elements, both requirements typical of NCR-sensitive transcription. These data demonstrate that regulated Gat1 production is more complex than previously recognized, with wild type versus truncated Gat1 proteins failing to be regulated in parallel. This is the first reported instance of Gln3 UAS GATA-dependent protein production failing to derepress in nitrogen poor conditions. A Gat1-lacZ ORF swap experiment indicated sequence(s) responsible for the nonparallel production are downstream of Gat1 leucine 61.

Original languageEnglish (US)
Pages (from-to)2918-2933
Number of pages16
JournalJournal of Biological Chemistry
Volume289
Issue number5
DOIs
StatePublished - Jan 31 2014

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Catabolite Repression
Protein Isoforms
Nitrogen
Transcription
Methionine
Cloning
Glutamine
Proline
Leucine
Open Reading Frames
Organism Cloning
Proteins
Western Blotting

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Constitutive and nitrogen catabolite repression-sensitive production of Gat1 isoforms. / Rai, Rajendra; Tate, Jennifer J.; Georis, Isabelle; Dubois, Evelyne; Cooper, Terrance.

In: Journal of Biological Chemistry, Vol. 289, No. 5, 31.01.2014, p. 2918-2933.

Research output: Contribution to journalArticle

Rai, Rajendra ; Tate, Jennifer J. ; Georis, Isabelle ; Dubois, Evelyne ; Cooper, Terrance. / Constitutive and nitrogen catabolite repression-sensitive production of Gat1 isoforms. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 5. pp. 2918-2933.
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