Constitutively active PKA regulates neuronal acetylcholine release and contractility of Guinea pig urinary bladder smooth muscle

Wenkuan Xin, Ning Li, Vitor S. Fernandes, Georgi Petkov

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Autonomic and somatic motor neurons that innervate the urinary bladder and urethra control the highly coordinated functions of the lower urinary tract, the storage, and the emptying of urine. ACh is the primary excitatory neurotransmitter in the bladder. Here, we aimed to determine whether PKA regulates neuronal ACh release and related nerve-evoked detrusor smooth muscle (DSM) contractions in the guinea pig urinary bladder. Isometric DSM tension recordings were used to measure spontaneous phasic and electrical field stimulation (EFS)- and carbachol-induced DSM contractions with a combination of pharmacological tools. The colorimetric method was used to measure ACh released by the parasympathetic nerves in DSM isolated strips. The pharmacological inhibition of PKA with H-89 (10 μM) increased the spontaneous phasic contractions, whereas it attenuated the EFS-induced DSM contractions. Intriguingly, H-89 (10 μM) attenuated the (primary) cholinergic component, whereas it simultaneously increased the (sec-ondary) purinergic component of the nerve-evoked contractions in DSM isolated strips. The acetylcholinesterase inhibitor, eserine (10 μM), increased EFS-induced DSM contractions, and the subsequent addition of H-89 attenuated the contractions. H-89 (10 μM) significantly increased DSM phasic contractions induced by the cholinergic agonist carbachol. The inhibition of PKA decreased the neuronal release of ACh in DSM tissues. This study revealed that PKA-mediated signaling pathways differentially regulate nerve-evoked and spontaneous phasic contractions of guinea pig DSM. Constitutively active PKA in the bladder nerves controls synaptic ACh release, thus regulating the nerve-evoked DSM contractions, whereas PKA in DSM cells controls the spontaneous phasic contractility.

Original languageEnglish (US)
Pages (from-to)F1377-F1384
JournalAmerican Journal of Physiology - Renal Physiology
Volume310
Issue number11
DOIs
StatePublished - Jun 1 2016

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Acetylcholine
Smooth Muscle
Guinea Pigs
Urinary Bladder
Muscle Contraction
Electric Stimulation
Carbachol
Pharmacology
Cholinergic Agonists
Muscle Tonus
Physostigmine
Cholinesterase Inhibitors
Motor Neurons
Urethra
Urinary Tract
Cholinergic Agents
Smooth Muscle Myocytes
Neurotransmitter Agents
Urine
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Urology

Cite this

Constitutively active PKA regulates neuronal acetylcholine release and contractility of Guinea pig urinary bladder smooth muscle. / Xin, Wenkuan; Li, Ning; Fernandes, Vitor S.; Petkov, Georgi.

In: American Journal of Physiology - Renal Physiology, Vol. 310, No. 11, 01.06.2016, p. F1377-F1384.

Research output: Contribution to journalArticle

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