Construction of a BAC contig for a 3 cM biologically significant region of mouse chromosome 1

Weikuan Gu, X. M. Li, B. Edderkaoui, D. D. Strong, K. H.W. Lau, W. G. Beamer, L. R. Donahae, S. Mohan, D. J. Baylink

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

One QTL and genes and phenotypes have been localized in the region between 92 cM and 95 cM of mouse chromosome 1. The QTL locus contributes to approximately 40% of the variation of the peak bone density between C57BL/6J (B6) and CAST/EiJ (CAST) strains. Other loci located in this chromosomal region include a neural tube defect mutant loop-tail (Lp), a lymphocyte-stimulating determinant (Lsd), and the Transgelin 2 (Tagln 2). The human chromosome region homologous to this region is 1q21-23, which also contains a QTL locus for high bone mineral density (BMD). Furthermore, it has been reported that this region may have duplicated several times in the mouse genome. Therefore, genomic sequencing of this region will provide important information for mouse genome structure, for positional cloning of mouse genes, and for the study of human homologous genes. In order to provide a suitable template for genomic sequencing by the NIH-sponsored genomic centers, we have constructed a BAC contig of this region using the RPCI-23 library. We have also identified the currently available mouse genomic sequences localized in our BAC contig. Further analysis of these sequences and BAC clones indicated a high frequency of repetitive sequences within this chromosomal area. This region also contains L1 retrotransposon sequences, providing a potential mechanism for the repetitive sequences described in the literature.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalGenetica
Volume114
Issue number1
DOIs
StatePublished - Apr 18 2002

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Chromosomes, Human, Pair 1
chromosomes
mice
genomics
quantitative trait loci
repetitive sequences
Nucleic Acid Repetitive Sequences
bone density
Bone Density
loci
Genome
Genes
neural tube defects
Retroelements
genome
genes
Neural Tube Defects
retrotransposons
Human Chromosomes
Libraries

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

Cite this

Gu, W., Li, X. M., Edderkaoui, B., Strong, D. D., Lau, K. H. W., Beamer, W. G., ... Baylink, D. J. (2002). Construction of a BAC contig for a 3 cM biologically significant region of mouse chromosome 1. Genetica, 114(1), 1-9. https://doi.org/10.1023/A:1014609809681

Construction of a BAC contig for a 3 cM biologically significant region of mouse chromosome 1. / Gu, Weikuan; Li, X. M.; Edderkaoui, B.; Strong, D. D.; Lau, K. H.W.; Beamer, W. G.; Donahae, L. R.; Mohan, S.; Baylink, D. J.

In: Genetica, Vol. 114, No. 1, 18.04.2002, p. 1-9.

Research output: Contribution to journalArticle

Gu, W, Li, XM, Edderkaoui, B, Strong, DD, Lau, KHW, Beamer, WG, Donahae, LR, Mohan, S & Baylink, DJ 2002, 'Construction of a BAC contig for a 3 cM biologically significant region of mouse chromosome 1', Genetica, vol. 114, no. 1, pp. 1-9. https://doi.org/10.1023/A:1014609809681
Gu, Weikuan ; Li, X. M. ; Edderkaoui, B. ; Strong, D. D. ; Lau, K. H.W. ; Beamer, W. G. ; Donahae, L. R. ; Mohan, S. ; Baylink, D. J. / Construction of a BAC contig for a 3 cM biologically significant region of mouse chromosome 1. In: Genetica. 2002 ; Vol. 114, No. 1. pp. 1-9.
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