Construction of a bioluminescence reporter plasmid for Francisella tularensis

Xiaowen R. Bina, Mark Miller, James E. Bina

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A. Francisella tularensis shuttle vector that constitutively expresses the Photorhabdus luminescens lux operon in type A and type B strains of F. tularensis was constructed. The bioluminescence reporter plasmid was introduced into the live vaccine strain of F. tularensis and used to follow F. tularensis growth in a murine intranasal challenge model in real-time by bioluminescence imaging. The results show that the new bioluminescence reporter plasmid represents a useful tool for tularemia research that is suitable for following F. tularensis growth in both in vitro and in vivo model systems.

Original languageEnglish (US)
Pages (from-to)156-161
Number of pages6
JournalPlasmid
Volume64
Issue number3
DOIs
StatePublished - Nov 1 2010

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Francisella tularensis
Plasmids
Photorhabdus
Tularemia
Genetic Vectors
Operon
Growth
Vaccines
Research

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Construction of a bioluminescence reporter plasmid for Francisella tularensis. / Bina, Xiaowen R.; Miller, Mark; Bina, James E.

In: Plasmid, Vol. 64, No. 3, 01.11.2010, p. 156-161.

Research output: Contribution to journalArticle

Bina, Xiaowen R. ; Miller, Mark ; Bina, James E. / Construction of a bioluminescence reporter plasmid for Francisella tularensis. In: Plasmid. 2010 ; Vol. 64, No. 3. pp. 156-161.
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