Continuous exposure of airway epithelial cells to hydrogen peroxide

Protection by KGF

Kenneth E. Chapman, Christopher Waters, William M. Miller

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) increase permeability in the airway epithelium. Extended periods of oxidant exposure may be experienced by those suffering from chronic inflammation of the lungs, receiving supplemental oxygen, or living in areas with high levels of air pollution. We studied the effects of long-term, continuous exposure to hydrogen peroxide (H2O2) on the trans-epithelial electrical resistance (TER) across cultured monolayers of a transformed cell line of human bronchial epithelial cells, 16HBE14o-(16HBE). A TER perfusion system was employed to continuously monitor the TER without disturbing the tissue model. The TER decreased in a dose-dependent manner with increasing concentrations of H2O2 (0.1, 0.5, and 1.0 mM), regardless of pre-incubation conditions. Cell cultures pre-treated with 50 ng/ml keratinocyte growth factor (KGF) showed a significant delay in oxidant-induced TER decreases caused by 0.1 mM H2O2. Exposure to 0.1 mM H2O2 for 350 min led to disruption of tight junction proteins, ZO-1 and occludin, but KGF treatment prevented this damage. The recovery of epithelial barrier function after exposure to oxidants was also studied. Tissue models exposed to 0.5 mM H2O2 for 25 min showed complete recovery of TER after 20 h, independent of culture pre-treatment. In contrast, KGF pre-incubation enhanced the recovery of 16HBE cultures exposed for 50 min to 0.5 mM H2 O2.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalJournal of Cellular Physiology
Volume192
Issue number1
DOIs
StatePublished - Jun 13 2002

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Fibroblast Growth Factor 7
Acoustic impedance
Electric Impedance
Hydrogen Peroxide
Epithelial Cells
Oxidants
Recovery
Zonula Occludens-1 Protein
Tissue
Occludin
Transformed Cell Line
Air Pollution
Air pollution
Cell culture
Monolayers
Permeability
Reactive Oxygen Species
Pneumonia
Epithelium
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Continuous exposure of airway epithelial cells to hydrogen peroxide : Protection by KGF. / Chapman, Kenneth E.; Waters, Christopher; Miller, William M.

In: Journal of Cellular Physiology, Vol. 192, No. 1, 13.06.2002, p. 71-80.

Research output: Contribution to journalArticle

Chapman, Kenneth E. ; Waters, Christopher ; Miller, William M. / Continuous exposure of airway epithelial cells to hydrogen peroxide : Protection by KGF. In: Journal of Cellular Physiology. 2002 ; Vol. 192, No. 1. pp. 71-80.
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