Continuous measurement of cerebral cortical blood flow by Laser-Doppler flowmetry in a rat stroke model

U. Dirnagl, B. Kaplan, Michael Jacewicz, W. Pulsinelli

Research output: Contribution to journalArticle

393 Citations (Scopus)

Abstract

Laser-Doppler flowmetry (LDF), a new method allowing instantaneous, continuous, and noninvasive measurements of microcirculatory blood flow in a small tissue sample, was evaluated for its accuracy in monitoring regional cerebral blood flow (rCBF) in the cortical microcirculation after focal cerebral ischemia. Wistar and spontaneously hypertensive rats (SHR, n = 19) were subjected to permanent occlusion of the middle cerebral and common carotid arteries. Absolute rCBF in a tissue sample of the ischemic hemisphere was measured autoradiographically with [14C]iodoantipyrine as a tracer and compared to rCBF measured by LDF. Additionally, the percent change in rCBF between baseline and ischemic values was compared for both methods. Absolute rCBF values recorded with LDF correlated poorly (r = 0.54) with [14C]iodoantipyrine measurements. In contrast, LDF readings expressed as a percentage of ischemic vs. preocclusion readings (relative LDF readings) correlated very well (r = 0.91) with the percent change in [14C]iodoantipyrine measurements. We conclude that LDF does not provide accurate measurements of absolute rCBF values but this method allows accurate measurements of changes in rCBF due to induction of focal cerebral ischemia.

Original languageEnglish (US)
Pages (from-to)589-596
Number of pages8
JournalJournal of Cerebral Blood Flow and Metabolism
Volume9
Issue number5
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Cerebrovascular Circulation
Laser-Doppler Flowmetry
Regional Blood Flow
Stroke
Reading
Brain Ischemia
Common Carotid Artery
Inbred SHR Rats
Microcirculation

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Continuous measurement of cerebral cortical blood flow by Laser-Doppler flowmetry in a rat stroke model. / Dirnagl, U.; Kaplan, B.; Jacewicz, Michael; Pulsinelli, W.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 9, No. 5, 01.01.1989, p. 589-596.

Research output: Contribution to journalArticle

@article{cc8b793132bf4379887b34f1b18f7a91,
title = "Continuous measurement of cerebral cortical blood flow by Laser-Doppler flowmetry in a rat stroke model",
abstract = "Laser-Doppler flowmetry (LDF), a new method allowing instantaneous, continuous, and noninvasive measurements of microcirculatory blood flow in a small tissue sample, was evaluated for its accuracy in monitoring regional cerebral blood flow (rCBF) in the cortical microcirculation after focal cerebral ischemia. Wistar and spontaneously hypertensive rats (SHR, n = 19) were subjected to permanent occlusion of the middle cerebral and common carotid arteries. Absolute rCBF in a tissue sample of the ischemic hemisphere was measured autoradiographically with [14C]iodoantipyrine as a tracer and compared to rCBF measured by LDF. Additionally, the percent change in rCBF between baseline and ischemic values was compared for both methods. Absolute rCBF values recorded with LDF correlated poorly (r = 0.54) with [14C]iodoantipyrine measurements. In contrast, LDF readings expressed as a percentage of ischemic vs. preocclusion readings (relative LDF readings) correlated very well (r = 0.91) with the percent change in [14C]iodoantipyrine measurements. We conclude that LDF does not provide accurate measurements of absolute rCBF values but this method allows accurate measurements of changes in rCBF due to induction of focal cerebral ischemia.",
author = "U. Dirnagl and B. Kaplan and Michael Jacewicz and W. Pulsinelli",
year = "1989",
month = "1",
day = "1",
doi = "10.1038/jcbfm.1989.84",
language = "English (US)",
volume = "9",
pages = "589--596",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Continuous measurement of cerebral cortical blood flow by Laser-Doppler flowmetry in a rat stroke model

AU - Dirnagl, U.

AU - Kaplan, B.

AU - Jacewicz, Michael

AU - Pulsinelli, W.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Laser-Doppler flowmetry (LDF), a new method allowing instantaneous, continuous, and noninvasive measurements of microcirculatory blood flow in a small tissue sample, was evaluated for its accuracy in monitoring regional cerebral blood flow (rCBF) in the cortical microcirculation after focal cerebral ischemia. Wistar and spontaneously hypertensive rats (SHR, n = 19) were subjected to permanent occlusion of the middle cerebral and common carotid arteries. Absolute rCBF in a tissue sample of the ischemic hemisphere was measured autoradiographically with [14C]iodoantipyrine as a tracer and compared to rCBF measured by LDF. Additionally, the percent change in rCBF between baseline and ischemic values was compared for both methods. Absolute rCBF values recorded with LDF correlated poorly (r = 0.54) with [14C]iodoantipyrine measurements. In contrast, LDF readings expressed as a percentage of ischemic vs. preocclusion readings (relative LDF readings) correlated very well (r = 0.91) with the percent change in [14C]iodoantipyrine measurements. We conclude that LDF does not provide accurate measurements of absolute rCBF values but this method allows accurate measurements of changes in rCBF due to induction of focal cerebral ischemia.

AB - Laser-Doppler flowmetry (LDF), a new method allowing instantaneous, continuous, and noninvasive measurements of microcirculatory blood flow in a small tissue sample, was evaluated for its accuracy in monitoring regional cerebral blood flow (rCBF) in the cortical microcirculation after focal cerebral ischemia. Wistar and spontaneously hypertensive rats (SHR, n = 19) were subjected to permanent occlusion of the middle cerebral and common carotid arteries. Absolute rCBF in a tissue sample of the ischemic hemisphere was measured autoradiographically with [14C]iodoantipyrine as a tracer and compared to rCBF measured by LDF. Additionally, the percent change in rCBF between baseline and ischemic values was compared for both methods. Absolute rCBF values recorded with LDF correlated poorly (r = 0.54) with [14C]iodoantipyrine measurements. In contrast, LDF readings expressed as a percentage of ischemic vs. preocclusion readings (relative LDF readings) correlated very well (r = 0.91) with the percent change in [14C]iodoantipyrine measurements. We conclude that LDF does not provide accurate measurements of absolute rCBF values but this method allows accurate measurements of changes in rCBF due to induction of focal cerebral ischemia.

UR - http://www.scopus.com/inward/record.url?scp=0024439730&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024439730&partnerID=8YFLogxK

U2 - 10.1038/jcbfm.1989.84

DO - 10.1038/jcbfm.1989.84

M3 - Article

VL - 9

SP - 589

EP - 596

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 5

ER -