Contribution of adenosine to isoproterenol-stimulated prostacyclin production in rabbit heart

C. Cano, Z. Qureshi, S. Carter, Kafait Malik

Research output: Contribution to journalArticle

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Abstract

This study investigated adenosine's contribution to isoproterenol- stimulated prostacyclin production, measured as 6-ketoprostaglandin F(1α) (6-keto-PGF(α)) output, and mechanical function in the isolated rabbit heart perfused with Krebs-Henseleit buffer. The isoproterenol-induced increase in 6-keto-PGF(1α) was diminished by adenosine (10 μM), the A1 receptor antagonist 1,3-dipropyl, 8-cyclopentylxanthine (DPCPX 0.06 μM), and the A2 receptor agonist CGS-21680 (0.6 μM); CGS-21680 did not decrease heart rate (HR) or myocardial contractility (dP/dt(max)). The isoproterenol-induced increase in 6-keto-PGF(1α) was potentiated by the A1 receptor agonist 1- deaza,2-chloro,N6-cyclopentyladenosine (DCCA, 0.6 μM) and the A2 receptor antagonist 3,7-dimethyl,1-propargylxanthine (DMPX, 6 μM). The isoproterenol- induced increase in dP/dt(max) and HR was diminished by adenosine, DCCA, and DMPX. DPCPX enhanced dP/dt(max) and HR and prevented the decrease by adenosine and DCCA of the isoproterenol-induced increase in HR and dP/dt(max); the increase by DCCA but not the decrease by adenosine in 6-keto- PGF(1α) output was abolished. DMPX abolished the effect of adenosine and CGS-21680 to reduce isoproterenol-stimulated 6-keto-PGF(1α). These data suggest that adenosine generated in response to isoproterenol attenuates its effect on HR and dP/dt(max) through A1 receptors and on prostacyclin synthesis via A2 receptors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume263
Issue number1 32-1
StatePublished - Jan 1 1992

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Epoprostenol
Isoproterenol
Adenosine
Rabbits
Prostaglandins F
Heart Rate
varespladib methyl
Epoprostenol Receptors
3,7-dimethyl-1-propargylxanthine
2-(4-(2-carboxyethyl)phenethylamino)-5'-N-ethylcarboxamidoadenosine
1,3-dipropyl-8-cyclopentylxanthine

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Contribution of adenosine to isoproterenol-stimulated prostacyclin production in rabbit heart. / Cano, C.; Qureshi, Z.; Carter, S.; Malik, Kafait.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 263, No. 1 32-1, 01.01.1992.

Research output: Contribution to journalArticle

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