Contribution of Muller cells toward the regulation of photoreceptor outer segment assembly

Monica Jablonski, Xiaofei Wang, Iannaccone Alessandro

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The assembly of photoreceptor outer segments into stacked discs is a complicated process, the precise regulation of which remains a mystery. It is known that the integrity of the outer segment is heavily dependent upon surrounding cell types including the retinal pigment epithelium and Muller cells; however the role played by Muller cells within this photoreceptor-specific process has not been fully explored. Using an RPE-deprived but otherwise intact Xenopus laevis eye rudiment preparation, we reveal that Muller cell involvement in outer segment assembly is dependent upon the stimulus provided to the retina. Pigment epithelium-derived factor is able to support proper membrane folding after inhibition of Muller cell metabolism by alpha-aminoadipic acid, while isopropyl beta-D-thiogalactoside, a permissive glycan, requires intact Muller cell function. These results demonstrate that both intrinsic and extrinsic redundant mechanisms exist to support the ability of photoreceptors to properly assemble their outer segments. Our study further suggests that the receptor for pigment epithelium-derived factor resides in photoreceptors themselves while that for permissive glycans is likely localized to Muller cells, which in turn communicate with photoreceptors to promote proper membrane assembly.

Original languageEnglish (US)
Pages (from-to)291-296
Number of pages6
JournalNeuron Glia Biology
Volume1
Issue number3
DOIs
StatePublished - Jan 1 2004

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Ependymoglial Cells
Polysaccharides
2-Aminoadipic Acid
Thiogalactosides
Membranes
Retinal Pigment Epithelium
Xenopus laevis
Retina

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Contribution of Muller cells toward the regulation of photoreceptor outer segment assembly. / Jablonski, Monica; Wang, Xiaofei; Alessandro, Iannaccone.

In: Neuron Glia Biology, Vol. 1, No. 3, 01.01.2004, p. 291-296.

Research output: Contribution to journalArticle

Jablonski, Monica ; Wang, Xiaofei ; Alessandro, Iannaccone. / Contribution of Muller cells toward the regulation of photoreceptor outer segment assembly. In: Neuron Glia Biology. 2004 ; Vol. 1, No. 3. pp. 291-296.
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