Contribution of PKC to β-hexosaminidase-induced airway smooth muscle proliferation

D. Betty Lew, Eric R. Brown, B. Kinard Dempsey, Harold M. Wright, Kafait Malik

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

β-Hexosaminidases (Hex) A and B promote mitogenesis via airway smooth muscle (ASM) mannose receptor. The objective of this study was to elucidate the contribution of protein kinase C (PKC) in Hex-induced mitogenesis in ASM cells (ASMC). Exposure of ASMC to Hex caused increases in both the calcium- dependent and the calcium-independent PKC activities. Both downregulation of PKC and PKC inhibitors staurosporine and calphostin C diminished Hex-induced DNA synthesis and cell number. Hex-induced DNA synthesis was enhanced by a diacylglycerol kinase inhibitor, R-59022, which was blocked by calphostin C. These data suggest that activation of PKC in part mediates Hex-induced mitogenesis in ASMC.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume272
Issue number4 16-4
StatePublished - Apr 1 1997

Fingerprint

Hexosaminidases
Protein Kinase C
Smooth Muscle
R 59022
Hexosaminidase B
Hexosaminidase A
Diacylglycerol Kinase
Staurosporine
Protein C Inhibitor
DNA
Protein Kinase Inhibitors
Smooth Muscle Myocytes
Down-Regulation
Cell Count
Calcium

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Contribution of PKC to β-hexosaminidase-induced airway smooth muscle proliferation. / Lew, D. Betty; Brown, Eric R.; Dempsey, B. Kinard; Wright, Harold M.; Malik, Kafait.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 272, No. 4 16-4, 01.04.1997.

Research output: Contribution to journalArticle

@article{73e187cec69e49ce916acb46ffc5e2a2,
title = "Contribution of PKC to β-hexosaminidase-induced airway smooth muscle proliferation",
abstract = "β-Hexosaminidases (Hex) A and B promote mitogenesis via airway smooth muscle (ASM) mannose receptor. The objective of this study was to elucidate the contribution of protein kinase C (PKC) in Hex-induced mitogenesis in ASM cells (ASMC). Exposure of ASMC to Hex caused increases in both the calcium- dependent and the calcium-independent PKC activities. Both downregulation of PKC and PKC inhibitors staurosporine and calphostin C diminished Hex-induced DNA synthesis and cell number. Hex-induced DNA synthesis was enhanced by a diacylglycerol kinase inhibitor, R-59022, which was blocked by calphostin C. These data suggest that activation of PKC in part mediates Hex-induced mitogenesis in ASMC.",
author = "Lew, {D. Betty} and Brown, {Eric R.} and Dempsey, {B. Kinard} and Wright, {Harold M.} and Kafait Malik",
year = "1997",
month = "4",
day = "1",
language = "English (US)",
volume = "272",
journal = "American Journal of Physiology - Lung Cellular and Molecular Physiology",
issn = "1040-0605",
publisher = "American Physiological Society",
number = "4 16-4",

}

TY - JOUR

T1 - Contribution of PKC to β-hexosaminidase-induced airway smooth muscle proliferation

AU - Lew, D. Betty

AU - Brown, Eric R.

AU - Dempsey, B. Kinard

AU - Wright, Harold M.

AU - Malik, Kafait

PY - 1997/4/1

Y1 - 1997/4/1

N2 - β-Hexosaminidases (Hex) A and B promote mitogenesis via airway smooth muscle (ASM) mannose receptor. The objective of this study was to elucidate the contribution of protein kinase C (PKC) in Hex-induced mitogenesis in ASM cells (ASMC). Exposure of ASMC to Hex caused increases in both the calcium- dependent and the calcium-independent PKC activities. Both downregulation of PKC and PKC inhibitors staurosporine and calphostin C diminished Hex-induced DNA synthesis and cell number. Hex-induced DNA synthesis was enhanced by a diacylglycerol kinase inhibitor, R-59022, which was blocked by calphostin C. These data suggest that activation of PKC in part mediates Hex-induced mitogenesis in ASMC.

AB - β-Hexosaminidases (Hex) A and B promote mitogenesis via airway smooth muscle (ASM) mannose receptor. The objective of this study was to elucidate the contribution of protein kinase C (PKC) in Hex-induced mitogenesis in ASM cells (ASMC). Exposure of ASMC to Hex caused increases in both the calcium- dependent and the calcium-independent PKC activities. Both downregulation of PKC and PKC inhibitors staurosporine and calphostin C diminished Hex-induced DNA synthesis and cell number. Hex-induced DNA synthesis was enhanced by a diacylglycerol kinase inhibitor, R-59022, which was blocked by calphostin C. These data suggest that activation of PKC in part mediates Hex-induced mitogenesis in ASMC.

UR - http://www.scopus.com/inward/record.url?scp=0030938769&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030938769&partnerID=8YFLogxK

M3 - Article

VL - 272

JO - American Journal of Physiology - Lung Cellular and Molecular Physiology

JF - American Journal of Physiology - Lung Cellular and Molecular Physiology

SN - 1040-0605

IS - 4 16-4

ER -