Control of aquaporin 2 expression in collecting ducts of quail kidneys

Keith K. Lau, Yimu Yang, George Cook, Robert J. Wyatt, Hiroko Nishimura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Birds and mammals are the only vertebrates that can concentrate urine. Avian kidneys contain structurally primitive loopless nephrons and also more advanced looped nephrons, in the cortical and medullary regions, respectively. We have identified the gene sequence of an aquaporin 2 (AQP2)-homologue water channel in collecting ducts of kidneys from adult quail, Coturnix japonica. Although immunoreactive quail AQP2 (qAQP2) was found in both types of nephrons, the expression is enhanced more clearly in the medullary regions after water deprivation. We therefore hypothesized that regulation of qAQP2 expression in quail kidneys via antidiuretic hormone (ADH) may require more advanced nephron structure. In this study, we determined the expression of qAQP2 mRNA in tissues isolated from the cortical and medullary regions before and after water deprivation, by conventional reverse transcriptase-polymerase chain reaction (RT-PCR) and quantitative real-time PCR. In both normally hydrated and water-deprived groups, qAQP2 mRNA levels in the medullary regions were significantly higher (P < 0.01) than in the cortical regions. In medullary areas, qAQP2 mRNA levels (real-time PCR normalized with 18S) were significantly higher (P < 0.01, ANOVA) after water deprivation (1.09 ± 0.10) than in normally hydrated controls (0.46 ± 0.08). In cortical areas, qAQP2 mRNA levels were also higher after water deprivation (0.37 ± 0.05) than in controls (0.11 ± 0.02). qAQP2 mRNA signals determined by in situ hybridization of digoxigenin-labeled riboprobe were also enhanced after water deprivation in both cortical and medullary collecting ducts. The results suggest that, contrary to our hypothesis, the endogenous production of ADH by water deprivation stimulates qAQP2 mRNA in both loopless and looped nephrons.

Original languageEnglish (US)
Pages (from-to)288-294
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume160
Issue number3
DOIs
StatePublished - Jan 1 2009

Fingerprint

Collecting Kidney Tubules
Aquaporin 2
Quail
aquaporins
quails
Water Deprivation
water deprivation
kidneys
nephrons
Nephrons
Messenger RNA
vasopressin
Vasopressins
Kidney
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Digoxigenin
Coturnix
digoxigenin
Reverse Transcriptase Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Endocrinology

Cite this

Control of aquaporin 2 expression in collecting ducts of quail kidneys. / Lau, Keith K.; Yang, Yimu; Cook, George; Wyatt, Robert J.; Nishimura, Hiroko.

In: General and Comparative Endocrinology, Vol. 160, No. 3, 01.01.2009, p. 288-294.

Research output: Contribution to journalArticle

Lau, Keith K. ; Yang, Yimu ; Cook, George ; Wyatt, Robert J. ; Nishimura, Hiroko. / Control of aquaporin 2 expression in collecting ducts of quail kidneys. In: General and Comparative Endocrinology. 2009 ; Vol. 160, No. 3. pp. 288-294.
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