Controlled release of oxytetracycline in sheep

Yichun Sun, Yingxu Peng, Napasinee Aksornkoae, James R. Johnson, J. Gregg Boring, Daniel Scruggs, Robert C. Cooper, Steven Laizure, Atul J. Shukla

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A novel biodegradable injectable formulation of oxytetracycline (OTC) was administered subcutaneously to sheep at a dose of 40 mg/kg. Blood samples were collected from the jugular vein at predetermined time intervals. The concentration of OTC in plasma was analyzed by an HPLC method. The concentrations of OTC in plasma were maintained at or above 0.5 μg/ml (minimum inhibitory concentration) for approximately 6 days. The pharmacokinetic parameters of OTC in sheep were also determined by monitoring the plasma concentration of OTC after a single intravenous injection of a commercially available OTC formulation at 10 mg/kg body weight. The in vivo release profiles of OTC from the biodegradable injectable formulations in sheep were determined from the plasma concentration time profiles by the deconvolution method using PCDCON software. The in vitro release of OTC from the biodegradable injectable formulation was tested in phosphate buffer (pH 7.4), containing 0.686% w/v of sodium sulfite as antioxidant. The correlation between the in vitro and in vivo release of OTC from the injectable formulation was also evaluated. The results of the in vivo evaluation of the formulation in sheep indicated that a controlled release biodegradable injectable dosage form of OTC for food animals is feasible.

Original languageEnglish (US)
Pages (from-to)125-134
Number of pages10
JournalJournal of Controlled Release
Volume85
Issue number1-3
DOIs
StatePublished - Dec 13 2002

Fingerprint

Oxytetracycline
Sheep
Injections
Jugular Veins
Dosage Forms
Microbial Sensitivity Tests
Intravenous Injections
Buffers
Software
Pharmacokinetics
Antioxidants
Phosphates
High Pressure Liquid Chromatography
Body Weight
Food

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

Sun, Y., Peng, Y., Aksornkoae, N., Johnson, J. R., Boring, J. G., Scruggs, D., ... Shukla, A. J. (2002). Controlled release of oxytetracycline in sheep. Journal of Controlled Release, 85(1-3), 125-134. https://doi.org/10.1016/S0168-3659(02)00286-9

Controlled release of oxytetracycline in sheep. / Sun, Yichun; Peng, Yingxu; Aksornkoae, Napasinee; Johnson, James R.; Boring, J. Gregg; Scruggs, Daniel; Cooper, Robert C.; Laizure, Steven; Shukla, Atul J.

In: Journal of Controlled Release, Vol. 85, No. 1-3, 13.12.2002, p. 125-134.

Research output: Contribution to journalArticle

Sun, Y, Peng, Y, Aksornkoae, N, Johnson, JR, Boring, JG, Scruggs, D, Cooper, RC, Laizure, S & Shukla, AJ 2002, 'Controlled release of oxytetracycline in sheep', Journal of Controlled Release, vol. 85, no. 1-3, pp. 125-134. https://doi.org/10.1016/S0168-3659(02)00286-9
Sun Y, Peng Y, Aksornkoae N, Johnson JR, Boring JG, Scruggs D et al. Controlled release of oxytetracycline in sheep. Journal of Controlled Release. 2002 Dec 13;85(1-3):125-134. https://doi.org/10.1016/S0168-3659(02)00286-9
Sun, Yichun ; Peng, Yingxu ; Aksornkoae, Napasinee ; Johnson, James R. ; Boring, J. Gregg ; Scruggs, Daniel ; Cooper, Robert C. ; Laizure, Steven ; Shukla, Atul J. / Controlled release of oxytetracycline in sheep. In: Journal of Controlled Release. 2002 ; Vol. 85, No. 1-3. pp. 125-134.
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