Copper deficiency increases metallothionein-I mRNA content selectively in rat liver

Yan Chen, Jack T. Saari, Yujian Kang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A variety of inducers of metallothionein (MT) synthesis have been identified. Copper loading, in particular, has been shown to increase MT expression in the liver and other organs of rodents. In the present study, the effect of severe dietary copper deficiency on MT expression was determined. Weanling rats were fed a purified diet deficient in copper (0.4 μg/g of diet) or containing adequate copper (5.7 μg/g of diet) for 4 weeks. Mineral analysis revealed that copper concentrations were severely depressed in the liver, heart, and kidney of the animals fed the copper-deficient diet. The mRNA content for MT-I was markedly increased in the copper-deficient liver but not in the heart or kidney. The metallothionein protein concentration, however, was not elevated in either liver or heart and was decreased in the kidney of copper-deficient rats. The results demonstrate that copper deficiency enhances MT-I transcription selectively in the liver of rats. It also suggests that copper is not essential for MT transcription in rats.

Original languageEnglish (US)
Pages (from-to)572-576
Number of pages5
JournalThe Journal of Nutritional Biochemistry
Volume6
Issue number11
DOIs
StatePublished - Jan 1 1995

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Metallothionein
Liver
Rats
Copper
Messenger RNA
Nutrition
Diet
Transcription
Kidney
Minerals
Rodentia
Animals

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Copper deficiency increases metallothionein-I mRNA content selectively in rat liver. / Chen, Yan; Saari, Jack T.; Kang, Yujian.

In: The Journal of Nutritional Biochemistry, Vol. 6, No. 11, 01.01.1995, p. 572-576.

Research output: Contribution to journalArticle

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