Copper inhibition of hydrogen peroxide-induced hypertrophy in embryonic rat cardiac H9c2 cells

Yang Zhou, Youchun Jiang, Yujian Kang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Previous studies have shown that dietary copper deficiency causes cardiac hypertrophy and depression of vascular epithelial growth factor (VEGF) expression in mouse model. Copper replenishment in the diet reverses cardiac hypertrophy and restores VEGF expression. The present study was undertaken to specifically determine the role of VEGF in copper effect on cell hypertrophy. Embryonic rat cardiac H9c2 cells were exposed to hydrogen peroxide to develop hypertrophy, determined by increases in cell size and total protein content. Copper addition at 5 μM in cultures suppressed cell hypertrophy. In the presence of anti-VEGF antibody, copper inhibitory effect on cell hypertrophy was blunted, and VEGF alone mimicked the inhibitory effect of copper. The results thus demonstrated that VEGF is critically involved in copper inhibition of cell hypertrophy induced by hydrogen peroxide in the H9c2 cells.

Original languageEnglish (US)
Pages (from-to)385-389
Number of pages5
JournalExperimental Biology and Medicine
Volume232
Issue number3
StatePublished - Mar 1 2007
Externally publishedYes

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Hypertrophy
Hydrogen Peroxide
Rats
Copper
Blood Vessels
Intercellular Signaling Peptides and Proteins
Cardiomegaly
Nutrition
Cell Size
Cell culture
Cell Culture Techniques
Cells
Diet
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Copper inhibition of hydrogen peroxide-induced hypertrophy in embryonic rat cardiac H9c2 cells. / Zhou, Yang; Jiang, Youchun; Kang, Yujian.

In: Experimental Biology and Medicine, Vol. 232, No. 3, 01.03.2007, p. 385-389.

Research output: Contribution to journalArticle

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