Coronary vascular hyperpermeability and angiotensin II.

H. K. Reddy, H. Sigusch, G. Zhou, S. C. Tyagi, J. S. Janicki, Karl Weber

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Elevations in plasma angiotensin II (AngII) are associated with evidence of vascular hyperpermeability expressed as efflux of plasma macromolecules into the perivascular and interstitial space. This exudative response is followed by a series of fibrogenic events that lead to a perivascular fibrosis of involved vessels. Mediators of hyperpermeability and fibrogenesis are unknown. In dogs receiving intravenous AngII, hemodynamic factors (i.e., arterial hypertension or coronary venoconstriction) were discounted as being responsible for the rise in cardiac lymph-to-plasma protein ratio. Accordingly, we investigated the relationship between AngII-induced coronary hyperpermeability and the release of prostaglandin E2 (PGE2) and activation of the basement membrane degrading matrix metalloproteinase, gelatinase/type IV collagenase. In dogs, cardiac lymph was monitored over the course of a 90-minute intravenous infusion of either AngII (0.2 to 0.3 micrograms/kg/min; n = 8) or saline solution (n = 6). Lymph was examined at 30-minute intervals for the following: total protein (Lowry's method), albumin (sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)), plasma fibronectin (SDS-PAGE and enzyme-linked immunosorbent assay); PGE2 (radioimmunoassay) and gelatinase/type IV collagenase (zymography). In comparison with baseline we found a consistent rise in lymph flow (p = 0.02), total protein (p = 0.02), albumin, fibronectin, PGE2 (p = 0.03), and gelatinase/type IV collagenase (p = 0.019), which began after 30 minutes of AngII infusion. Similar trends were not observed in dogs receiving saline solution alone. We therefore conclude that AngII-induced coronary vascular hyperpermeability is associated with an early release of PGE2 and gelatinase.

Original languageEnglish (US)
Pages (from-to)307-315
Number of pages9
JournalJournal of Laboratory and Clinical Medicine
Volume126
Issue number3
StatePublished - Sep 1 1995
Externally publishedYes

Fingerprint

Angiotensin II
Blood Vessels
Gelatinases
Lymph
Dinoprostone
Collagenases
Dogs
Electrophoresis
Plasmas
Fibronectins
Sodium Chloride
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Albumins
Immunosorbents
Hemodynamics
Matrix Metalloproteinases
Macromolecules
Basement Membrane
Intravenous Infusions

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Reddy, H. K., Sigusch, H., Zhou, G., Tyagi, S. C., Janicki, J. S., & Weber, K. (1995). Coronary vascular hyperpermeability and angiotensin II. Journal of Laboratory and Clinical Medicine, 126(3), 307-315.

Coronary vascular hyperpermeability and angiotensin II. / Reddy, H. K.; Sigusch, H.; Zhou, G.; Tyagi, S. C.; Janicki, J. S.; Weber, Karl.

In: Journal of Laboratory and Clinical Medicine, Vol. 126, No. 3, 01.09.1995, p. 307-315.

Research output: Contribution to journalArticle

Reddy, HK, Sigusch, H, Zhou, G, Tyagi, SC, Janicki, JS & Weber, K 1995, 'Coronary vascular hyperpermeability and angiotensin II.', Journal of Laboratory and Clinical Medicine, vol. 126, no. 3, pp. 307-315.
Reddy HK, Sigusch H, Zhou G, Tyagi SC, Janicki JS, Weber K. Coronary vascular hyperpermeability and angiotensin II. Journal of Laboratory and Clinical Medicine. 1995 Sep 1;126(3):307-315.
Reddy, H. K. ; Sigusch, H. ; Zhou, G. ; Tyagi, S. C. ; Janicki, J. S. ; Weber, Karl. / Coronary vascular hyperpermeability and angiotensin II. In: Journal of Laboratory and Clinical Medicine. 1995 ; Vol. 126, No. 3. pp. 307-315.
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