Correlation of N-cadherin expression in high grade gliomas with tissue invasion

Kenichiro Asano, Christopher D. Duntsch, Qihong Zhou, James D. Weimar, Dwight Bordelon, Jon H. Robertson, Tayebeh Pourmotabbed

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Cadherins are Ca 2+ -dependent cell adhesion molecules that play an important role in tissue construction and morphogenesis in multicellular organisms. Over the last few years, reports have emerged in the literature describing the involvement of cadherins in tumor invasion and metastasis. Cadherins typically demonstrate up and down-regulation according to the biological needs of the tissue. Additionally, up-regulation of N-cadherin is thought to be important for tumor formation in early stages of tumor development. We studied N-cadherin in surgical specimens of patients with primary glioblastoma by microarray analysis and found that N-cadherin mRNA expression is up-regulated compared to normal brain. To study the effects of N-cadherin expression on invasion and metastasis in vitro and in vivo, we overexpressed N-cadherin in the rat C6 glioma cell line which normally has low levels of N-cadherin. We found that up-regulation of N-cadherin resulted in a slight decreased adhesion to type IV collagen, fibronectin, and laminin, but statistically significant decreased adhesion to type I collagen. Furthermore, increased expression of N-cadherin correlated with a dramatic decrease in invasive behavior in extracellular matrix invasion assays. We then proceeded to study these cell lines in vivo in a rat intracranial glioma model, and found that N-cadherin expression inversely correlated with invasion into surrounding tissues, irregular margins, and extracranial invasion. In summary, these data collectively demonstrate that N-cadherin levels are important in the malignant behavior of gliomas, and may serve as a prognostic indicator for patients with high-grade gliomas.

Original languageEnglish (US)
Pages (from-to)3-15
Number of pages13
JournalJournal of Neuro-Oncology
Volume70
Issue number1
DOIs
StatePublished - Oct 1 2004

Fingerprint

Cadherins
Glioma
Up-Regulation
Neoplasm Metastasis
Cell Line
Neoplasms
Collagen Type IV
Cell Adhesion Molecules
Laminin
Glioblastoma
Microarray Analysis
Collagen Type I
Morphogenesis
Fibronectins
Extracellular Matrix
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Oncology
  • Neurology
  • Clinical Neurology
  • Cancer Research

Cite this

Correlation of N-cadherin expression in high grade gliomas with tissue invasion. / Asano, Kenichiro; Duntsch, Christopher D.; Zhou, Qihong; Weimar, James D.; Bordelon, Dwight; Robertson, Jon H.; Pourmotabbed, Tayebeh.

In: Journal of Neuro-Oncology, Vol. 70, No. 1, 01.10.2004, p. 3-15.

Research output: Contribution to journalArticle

Asano, Kenichiro ; Duntsch, Christopher D. ; Zhou, Qihong ; Weimar, James D. ; Bordelon, Dwight ; Robertson, Jon H. ; Pourmotabbed, Tayebeh. / Correlation of N-cadherin expression in high grade gliomas with tissue invasion. In: Journal of Neuro-Oncology. 2004 ; Vol. 70, No. 1. pp. 3-15.
@article{c8814b6c4b634a6c801de22903d12e5d,
title = "Correlation of N-cadherin expression in high grade gliomas with tissue invasion",
abstract = "Cadherins are Ca 2+ -dependent cell adhesion molecules that play an important role in tissue construction and morphogenesis in multicellular organisms. Over the last few years, reports have emerged in the literature describing the involvement of cadherins in tumor invasion and metastasis. Cadherins typically demonstrate up and down-regulation according to the biological needs of the tissue. Additionally, up-regulation of N-cadherin is thought to be important for tumor formation in early stages of tumor development. We studied N-cadherin in surgical specimens of patients with primary glioblastoma by microarray analysis and found that N-cadherin mRNA expression is up-regulated compared to normal brain. To study the effects of N-cadherin expression on invasion and metastasis in vitro and in vivo, we overexpressed N-cadherin in the rat C6 glioma cell line which normally has low levels of N-cadherin. We found that up-regulation of N-cadherin resulted in a slight decreased adhesion to type IV collagen, fibronectin, and laminin, but statistically significant decreased adhesion to type I collagen. Furthermore, increased expression of N-cadherin correlated with a dramatic decrease in invasive behavior in extracellular matrix invasion assays. We then proceeded to study these cell lines in vivo in a rat intracranial glioma model, and found that N-cadherin expression inversely correlated with invasion into surrounding tissues, irregular margins, and extracranial invasion. In summary, these data collectively demonstrate that N-cadherin levels are important in the malignant behavior of gliomas, and may serve as a prognostic indicator for patients with high-grade gliomas.",
author = "Kenichiro Asano and Duntsch, {Christopher D.} and Qihong Zhou and Weimar, {James D.} and Dwight Bordelon and Robertson, {Jon H.} and Tayebeh Pourmotabbed",
year = "2004",
month = "10",
day = "1",
doi = "10.1023/B:NEON.0000040811.14908.f2",
language = "English (US)",
volume = "70",
pages = "3--15",
journal = "Journal of Neuro-Oncology",
issn = "0167-594X",
publisher = "Kluwer Academic Publishers",
number = "1",

}

TY - JOUR

T1 - Correlation of N-cadherin expression in high grade gliomas with tissue invasion

AU - Asano, Kenichiro

AU - Duntsch, Christopher D.

AU - Zhou, Qihong

AU - Weimar, James D.

AU - Bordelon, Dwight

AU - Robertson, Jon H.

AU - Pourmotabbed, Tayebeh

PY - 2004/10/1

Y1 - 2004/10/1

N2 - Cadherins are Ca 2+ -dependent cell adhesion molecules that play an important role in tissue construction and morphogenesis in multicellular organisms. Over the last few years, reports have emerged in the literature describing the involvement of cadherins in tumor invasion and metastasis. Cadherins typically demonstrate up and down-regulation according to the biological needs of the tissue. Additionally, up-regulation of N-cadherin is thought to be important for tumor formation in early stages of tumor development. We studied N-cadherin in surgical specimens of patients with primary glioblastoma by microarray analysis and found that N-cadherin mRNA expression is up-regulated compared to normal brain. To study the effects of N-cadherin expression on invasion and metastasis in vitro and in vivo, we overexpressed N-cadherin in the rat C6 glioma cell line which normally has low levels of N-cadherin. We found that up-regulation of N-cadherin resulted in a slight decreased adhesion to type IV collagen, fibronectin, and laminin, but statistically significant decreased adhesion to type I collagen. Furthermore, increased expression of N-cadherin correlated with a dramatic decrease in invasive behavior in extracellular matrix invasion assays. We then proceeded to study these cell lines in vivo in a rat intracranial glioma model, and found that N-cadherin expression inversely correlated with invasion into surrounding tissues, irregular margins, and extracranial invasion. In summary, these data collectively demonstrate that N-cadherin levels are important in the malignant behavior of gliomas, and may serve as a prognostic indicator for patients with high-grade gliomas.

AB - Cadherins are Ca 2+ -dependent cell adhesion molecules that play an important role in tissue construction and morphogenesis in multicellular organisms. Over the last few years, reports have emerged in the literature describing the involvement of cadherins in tumor invasion and metastasis. Cadherins typically demonstrate up and down-regulation according to the biological needs of the tissue. Additionally, up-regulation of N-cadherin is thought to be important for tumor formation in early stages of tumor development. We studied N-cadherin in surgical specimens of patients with primary glioblastoma by microarray analysis and found that N-cadherin mRNA expression is up-regulated compared to normal brain. To study the effects of N-cadherin expression on invasion and metastasis in vitro and in vivo, we overexpressed N-cadherin in the rat C6 glioma cell line which normally has low levels of N-cadherin. We found that up-regulation of N-cadherin resulted in a slight decreased adhesion to type IV collagen, fibronectin, and laminin, but statistically significant decreased adhesion to type I collagen. Furthermore, increased expression of N-cadherin correlated with a dramatic decrease in invasive behavior in extracellular matrix invasion assays. We then proceeded to study these cell lines in vivo in a rat intracranial glioma model, and found that N-cadherin expression inversely correlated with invasion into surrounding tissues, irregular margins, and extracranial invasion. In summary, these data collectively demonstrate that N-cadherin levels are important in the malignant behavior of gliomas, and may serve as a prognostic indicator for patients with high-grade gliomas.

UR - http://www.scopus.com/inward/record.url?scp=4444277616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444277616&partnerID=8YFLogxK

U2 - 10.1023/B:NEON.0000040811.14908.f2

DO - 10.1023/B:NEON.0000040811.14908.f2

M3 - Article

VL - 70

SP - 3

EP - 15

JO - Journal of Neuro-Oncology

JF - Journal of Neuro-Oncology

SN - 0167-594X

IS - 1

ER -