Corticotropin-Releasing Hormone Inhibits Nuclear Factor-κB Pathway in Human HaCaT Keratinocytes

Blazej Zbytek, Lawrence Pfeffer, Andrzej T. Slominski

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Treatment of human HaCaT keratinocytes with corticotropin-releasing hormone modulates cell proliferation and expression of inflammation markers. In this study we report that corticotropin-releasing hormone also inhibits nuclear factor-κB binding and transcriptional activity. Incubating cells in the absence of growth factors increased nuclear factor-κB activity; this effect was significantly attenuated by corticotropin-releasing hormone. Specifically, corticotropin-releasing hormone downregulated p50/p50 and p50/p65 dimers of nuclear factor-κB, diminished κB-driven CAT reporter gene activity and inhibited IκB-β degradation. Moreover, corticotropin-releasing hormone inhibited the transcription of the nuclear factor-κB responsive genes, interleukin-2 and heat shock protein 90.

Original languageEnglish (US)
Pages (from-to)1496-1499
Number of pages4
JournalJournal of Investigative Dermatology
Volume121
Issue number6
DOIs
StatePublished - Jan 1 2003

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Corticotropin-Releasing Hormone
Keratinocytes
Genes
HSP90 Heat-Shock Proteins
Cell proliferation
Transcription
Reporter Genes
Dimers
Interleukin-2
Intercellular Signaling Peptides and Proteins
Transcription Factors
Down-Regulation
Cell Proliferation
Inflammation
Degradation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Corticotropin-Releasing Hormone Inhibits Nuclear Factor-κB Pathway in Human HaCaT Keratinocytes. / Zbytek, Blazej; Pfeffer, Lawrence; Slominski, Andrzej T.

In: Journal of Investigative Dermatology, Vol. 121, No. 6, 01.01.2003, p. 1496-1499.

Research output: Contribution to journalArticle

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