Corticotropin-releasing hormone stimulates NF-κB in human epidermal keratinocytes

Blazej Zbytek, Lawrence Pfeffer, Andrzej T. Slominski

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Corticotropin-releasing hormone (CRH) has been shown to inhibit proliferation and modulate expression of inflammation markers in the epidermal cells. In the present study we report that CRH also stimulates nuclear factor-kappa B (NF-κB) activity. Incubation with CRH of human keratinocytes derived from primary cultures resulted in increased binding of DNA by NF-κB. CRH induced translocation of NF-κB subunit p65 from the cytoplasm to the nucleus and induced expression of κB-driven chloramphenicol acetyltransferase (CAT) reporter gene. NF-κB translocation was accompanied by degradation of the inhibitor of NF-κB α (IκB-α). Specificity of the CRH effect was demonstrated by the use of CRH-R antagonists antalarmin and α-helical CRH [9-41]. CRH-dependent stimulation of NF-κB activity is consistent with accumulated data about role of this neuropeptide in the regulation of local epidermal homeostasis.

Original languageEnglish (US)
JournalJournal of Endocrinology
Volume181
Issue number3
DOIs
StatePublished - Jan 1 2004

Fingerprint

NF-kappa B
Corticotropin-Releasing Hormone
Keratinocytes
Hormone Antagonists
Chloramphenicol O-Acetyltransferase
Neuropeptides
Reporter Genes
Cytoplasm
Homeostasis
Inflammation
DNA

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Corticotropin-releasing hormone stimulates NF-κB in human epidermal keratinocytes. / Zbytek, Blazej; Pfeffer, Lawrence; Slominski, Andrzej T.

In: Journal of Endocrinology, Vol. 181, No. 3, 01.01.2004.

Research output: Contribution to journalArticle

@article{242baa99a95c4507bda615884977ff09,
title = "Corticotropin-releasing hormone stimulates NF-κB in human epidermal keratinocytes",
abstract = "Corticotropin-releasing hormone (CRH) has been shown to inhibit proliferation and modulate expression of inflammation markers in the epidermal cells. In the present study we report that CRH also stimulates nuclear factor-kappa B (NF-κB) activity. Incubation with CRH of human keratinocytes derived from primary cultures resulted in increased binding of DNA by NF-κB. CRH induced translocation of NF-κB subunit p65 from the cytoplasm to the nucleus and induced expression of κB-driven chloramphenicol acetyltransferase (CAT) reporter gene. NF-κB translocation was accompanied by degradation of the inhibitor of NF-κB α (IκB-α). Specificity of the CRH effect was demonstrated by the use of CRH-R antagonists antalarmin and α-helical CRH [9-41]. CRH-dependent stimulation of NF-κB activity is consistent with accumulated data about role of this neuropeptide in the regulation of local epidermal homeostasis.",
author = "Blazej Zbytek and Lawrence Pfeffer and Slominski, {Andrzej T.}",
year = "2004",
month = "1",
day = "1",
doi = "10.1677/joe.0.181R001",
language = "English (US)",
volume = "181",
journal = "Journal of Endocrinology",
issn = "0022-0795",
publisher = "Society for Endocrinology",
number = "3",

}

TY - JOUR

T1 - Corticotropin-releasing hormone stimulates NF-κB in human epidermal keratinocytes

AU - Zbytek, Blazej

AU - Pfeffer, Lawrence

AU - Slominski, Andrzej T.

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Corticotropin-releasing hormone (CRH) has been shown to inhibit proliferation and modulate expression of inflammation markers in the epidermal cells. In the present study we report that CRH also stimulates nuclear factor-kappa B (NF-κB) activity. Incubation with CRH of human keratinocytes derived from primary cultures resulted in increased binding of DNA by NF-κB. CRH induced translocation of NF-κB subunit p65 from the cytoplasm to the nucleus and induced expression of κB-driven chloramphenicol acetyltransferase (CAT) reporter gene. NF-κB translocation was accompanied by degradation of the inhibitor of NF-κB α (IκB-α). Specificity of the CRH effect was demonstrated by the use of CRH-R antagonists antalarmin and α-helical CRH [9-41]. CRH-dependent stimulation of NF-κB activity is consistent with accumulated data about role of this neuropeptide in the regulation of local epidermal homeostasis.

AB - Corticotropin-releasing hormone (CRH) has been shown to inhibit proliferation and modulate expression of inflammation markers in the epidermal cells. In the present study we report that CRH also stimulates nuclear factor-kappa B (NF-κB) activity. Incubation with CRH of human keratinocytes derived from primary cultures resulted in increased binding of DNA by NF-κB. CRH induced translocation of NF-κB subunit p65 from the cytoplasm to the nucleus and induced expression of κB-driven chloramphenicol acetyltransferase (CAT) reporter gene. NF-κB translocation was accompanied by degradation of the inhibitor of NF-κB α (IκB-α). Specificity of the CRH effect was demonstrated by the use of CRH-R antagonists antalarmin and α-helical CRH [9-41]. CRH-dependent stimulation of NF-κB activity is consistent with accumulated data about role of this neuropeptide in the regulation of local epidermal homeostasis.

UR - http://www.scopus.com/inward/record.url?scp=3042808577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042808577&partnerID=8YFLogxK

U2 - 10.1677/joe.0.181R001

DO - 10.1677/joe.0.181R001

M3 - Article

VL - 181

JO - Journal of Endocrinology

JF - Journal of Endocrinology

SN - 0022-0795

IS - 3

ER -