Counting cells in sectioned material

a suite of techniques, tools, and tips.

Robert Williams, Christopher S. von Bartheld, Glenn D. Rosen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

This unit presents protocols to obtain accurate estimates of cell density and cell number in sectioned material by using a light microscope. The "optical disector" or "3-D counting method" is described, followed by Abercrombie's less commonly used two-section comparison (TSC) method. These basic protocols are accompanied by four support protocols: one for celloidin embedding, which renders superb morphology, one for point counting, which is important for volume measurements and is almost always used in conjunction with the disector or 3-D counting, one for handling the potential problem of z-axis distortion and the consequences that this error can have on density estimates and sampling tactics when using the disector, and finally, one that provides a guide for calibrating and verifying estimates obtained by counting methods.

Original languageEnglish (US)
JournalCurrent protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.]
VolumeChapter 1
StatePublished - Jan 1 2003

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Cell Count
Collodion
Light

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Counting cells in sectioned material : a suite of techniques, tools, and tips. / Williams, Robert; von Bartheld, Christopher S.; Rosen, Glenn D.

In: Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.], Vol. Chapter 1, 01.01.2003.

Research output: Contribution to journalArticle

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